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[Transformation of bone marrow cells in rodents by recombinant plasmid pBRSV].

作者信息

Kazakova T B, Shatov V A, Verbina I A, Tsymbalenko N V, Kravtsov V D

出版信息

Tsitologiia. 1986 Dec;28(12):1345-50.

PMID:3029925
Abstract

Bone marrow cells (mouse strain CBA/Ca and Syrian hamster cells) were transformed with pBRSV DNA containing T-antigen of the SV40 virus. The SV40 T-antigen in transformed cell was detected in 0.5% cases by immunofluorescence with specific antibodies. Extrachromosomal localization of recombinant DNA was shown by means of retransformation of E. coli cells with cytoplasmic spleen DNA from mice previously injected intravenously the transformed bone marrow cells.

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