[重组质粒pBRSV对啮齿动物骨髓细胞的转化作用]
[Transformation of bone marrow cells in rodents by recombinant plasmid pBRSV].
作者信息
Kazakova T B, Shatov V A, Verbina I A, Tsymbalenko N V, Kravtsov V D
出版信息
Tsitologiia. 1986 Dec;28(12):1345-50.
PMID:3029925
Abstract
Bone marrow cells (mouse strain CBA/Ca and Syrian hamster cells) were transformed with pBRSV DNA containing T-antigen of the SV40 virus. The SV40 T-antigen in transformed cell was detected in 0.5% cases by immunofluorescence with specific antibodies. Extrachromosomal localization of recombinant DNA was shown by means of retransformation of E. coli cells with cytoplasmic spleen DNA from mice previously injected intravenously the transformed bone marrow cells.
摘要
用含有SV40病毒T抗原的pBRSV DNA转化骨髓细胞（CBA/Ca小鼠品系和叙利亚仓鼠细胞）。通过用特异性抗体进行免疫荧光检测，在0.5%的案例中检测到转化细胞中的SV40 T抗原。通过用先前静脉注射转化骨髓细胞的小鼠的细胞质脾脏DNA对大肠杆菌细胞进行再转化，显示了重组DNA的染色体外定位。
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