Stĕpánek J, Salajka E, Zuffa A, Mensík J, Franz J
Vet Med (Praha). 1987 Feb;32(2):65-80.
The most frequent microbial causative agents of massive diarrheas in new-born calves kept on large cattle farms in the CSSR are rotaviruses, coronaviruses and enterotoxigenic strains of E. coli, manifesting themselves as complicated virus-bacterial infections. An inactivated polyvalent adjuvant vaccine has been developed for the prevention and specific prophylaxis of these enteral infections; the vaccine contains bovine rotavirus, bovine coronavirus and three enterotoxigenic serotypes of E. coli with protective antigen K 99. The rotavirus and coronavirus are propagated on the stable cellular line MDBK and inactivated with 0.2% formalin, the Escherichia strains are submersed in the MINCA culture medium during their cultivation and inactivated with 0.5% formalin. The vaccine was prepared as a blend of the same amounts of rotavirus and coronavirus and of such an amount of bacterin so that 1 ml of the vaccine will contain 10(9) bacteria. One part of oil adjuvant was added to five parts of the virus-bacterial blend and the blend was homogenized in the Ultraturax apparatus. The vaccine is to be used for immunization of pregnant cows and heifers; in these animals it induces the production of specific antibodies to all antigens contained in the vaccine. Its immunogenic effects were checked in 32 calves and 38 cows in the herds with the occurrence of diarrheas caused by both enteropathogenic viruses and enterotoxigenic escherichia. It was demonstrated that the inactivation did not influence in either of the viruses the process of inducing the production of specific antibodies, and the antibody response of the calves and heifers after application of 2 ml of complete inactivated vaccine was equally strong as after application of live vaccine containing only rotavirus and coronavirus. The level of the rotavirus antibodies increased on the average 30 times and 200 times, coronavirus antibodies twice and four times. The antibody response to coronavirus was negatively influenced by the relatively high levels of antibodies before vaccination. The antibody response to antigen K 99 was expressive in all cases.
捷克斯洛伐克大型养牛场中新生犊牛大规模腹泻最常见的微生物病原体是轮状病毒、冠状病毒和产肠毒素大肠杆菌菌株，表现为复杂的病毒-细菌感染。已开发出一种灭活多价佐剂疫苗用于预防和特异性预防这些肠道感染；该疫苗含有牛轮状病毒、牛冠状病毒和三种带有保护性抗原K 99的产肠毒素大肠杆菌血清型。轮状病毒和冠状病毒在稳定细胞系MDBK上繁殖，并用0.2%福尔马林灭活，大肠杆菌菌株在培养过程中浸入MINCA培养基并用0.5%福尔马林灭活。疫苗制备为相同量的轮状病毒和冠状病毒与一定量菌苗的混合物，使1毫升疫苗含有10⁹个细菌。将一份油佐剂加入五份病毒-细菌混合物中，并在超声破碎仪中均质化。该疫苗用于怀孕母牛和小母牛的免疫；在这些动物中，它可诱导产生针对疫苗中所含所有抗原的特异性抗体。在发生由肠道致病病毒和产肠毒素大肠杆菌引起腹泻的牛群中，对32头犊牛和38头母牛进行了其免疫原性效果检查。结果表明，灭活对两种病毒诱导产生特异性抗体的过程均无影响，给犊牛和小母牛接种2毫升完全灭活疫苗后的抗体反应与接种仅含轮状病毒和冠状病毒的活疫苗后的抗体反应同样强烈。轮状病毒抗体水平平均升高30倍和200倍，冠状病毒抗体升高两倍和四倍。接种前相对较高的抗体水平对冠状病毒抗体反应有负面影响。对抗原K 99的抗体反应在所有情况下都很明显。
