Oslovsky Vladimir E, Drenichev Mikhail S, Alexeev Cyril S, Solyev Pavel N, Esipov Roman S, Mikhailov Sergey N
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Curr Protoc Nucleic Acid Chem. 2018 Dec;75(1):e61. doi: 10.1002/cpnc.61. Epub 2018 Oct 9.
This unit describes an effective method for the preparation of natural cytokinins and their synthetic derivatives based on enzymatic cleavage of the N-glycosidic bond of N -substituted adenosine or O -substituted inosine derivatives in the presence of purine nucleoside phosphorylase (PNP) and Na HAsO . The arsenolysis reaction is irreversible due to the hydrolysis of the resulting α-D-ribose-1-arsenate. As a result, the desired products are formed in near-quantitative yields, as indicated by high-performance liquid chromatography (HPLC) analysis, and can easily be isolated. In the strategy used here, the ribose residue acts as a protective group. © 2018 by John Wiley & Sons, Inc.
本单元描述了一种有效的方法，用于制备天然细胞分裂素及其合成衍生物，该方法基于在嘌呤核苷磷酸化酶（PNP）和NaHAsO存在下，对N-取代腺苷或O-取代肌苷衍生物的N-糖苷键进行酶促裂解。由于生成的α-D-核糖-1-砷酸盐发生水解，砷解反应是不可逆的。结果，如高效液相色谱（HPLC）分析所示，所需产物以近乎定量的产率形成，并且可以很容易地分离出来。在这里使用的策略中，核糖残基起保护基团的作用。© 2018约翰威立国际出版公司。
