Department of Anatomy, Second Military Medical University, Shanghai, China.
Department of Health Administration, Second Military Medical University, Shanghai, China.
Anat Rec (Hoboken). 2018 Nov;301(11):1968-1976. doi: 10.1002/ar.23947. Epub 2018 Oct 19.
Pim-1 is a proto-oncogene which has been discovered to involve in cell proliferation, differentiation, and survival. In this study, we observed the expression of Pim-1 in neonatal and adult rat retina and the changes in rat retina following optic nerve crush (ONC) in order to explore the relationship between Pim-1 and the survival of retinal ganglion cells (RGC). We discovered that Pim-1 was distributed mainly in retinal pigment epithelial cells (RPE) and retinal ganglion cell layer (GCL) in normal newborn rats, and it appeared in RPE, cone rod cell layer and GCL in normal adult rats by immunohistochemistry. Our double immunofluorescent staining of Pim-1 and γ-synuclein further confirmed that Pim-1 was localized in 80% of RGC. Moreover, we found that the amount of Pim-1 mRNA and protein in adult rat retina was transiently increased after ONC and then decreased 2 weeks after ONC, and the expression level was lower than that of neonatal rat retina under all conditions. We also discovered that Pim-1 expression in GCL detected by immunohistochemistry was upregulated at Day 1 and Day 3 after ONC, but downregulated at Day 14 after ONC when the survival of RGC was decreased and the apoptotic cells in GCL were increased by hematoxylin-eosin staining, immunohistochemistry, and TUNEL detection. We suggest that the overexpression of Pim-1 in the RGC is related to the optic nerve repair while the low expression of Pim-1 in RGC may be associated with apoptosis and weak intrinsic regeneration ability of RGC. Anat Rec, 301:1968-1976, 2018. © 2018 Wiley Periodicals, Inc.
Pim-1 是一种原癌基因，它被发现参与细胞增殖、分化和存活。在这项研究中，我们观察了 Pim-1 在新生和成年大鼠视网膜中的表达以及视神经挤压（ONC）后大鼠视网膜的变化，以探讨 Pim-1 与视网膜神经节细胞（RGC）存活之间的关系。我们发现，Pim-1 在正常新生大鼠的视网膜色素上皮细胞（RPE）和视网膜神经节细胞层（GCL）中主要分布，在正常成年大鼠中通过免疫组化显示出在 RPE、视锥杆细胞层和 GCL 中的分布。我们对 Pim-1 和 γ-突触核蛋白的双重免疫荧光染色进一步证实 Pim-1 定位于 80%的 RGC。此外，我们发现 ONC 后成年大鼠视网膜中 Pim-1 mRNA 和蛋白的量短暂增加，然后在 ONC 后 2 周减少，并且在所有条件下其表达水平均低于新生大鼠视网膜。我们还发现，免疫组化检测到的 GCL 中 Pim-1 的表达在 ONC 后第 1 天和第 3 天上调，但在 ONC 后第 14 天下调，此时 RGC 的存活减少，GCL 中的凋亡细胞增加，通过苏木精-伊红染色、免疫组化和 TUNEL 检测证实。我们认为，RGC 中 Pim-1 的过表达与视神经修复有关，而 RGC 中 Pim-1 的低表达可能与 RGC 的凋亡和较弱的内在再生能力有关。解剖记录，301:1968-1976, 2018. © 2018 Wiley Periodicals, Inc.
