Department of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Ghana.
Centre for Medical Parasitology at Department of Immunology and Microbiology (ISIM), Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
PLoS One. 2018 Oct 9;13(10):e0204177. doi: 10.1371/journal.pone.0204177. eCollection 2018.
The lack of suitable animal models for the study of cytoadhesion of P. falciparum-infected erythrocytes (IEs) has necessitated in vitro studies employing a range of cell lines of either human tumour origin (e.g., BeWo and C32 cells) or non-human origin (e.g., CHO cells). Of the human cells available, many were isolated from adults, or derived from a pool of donors (e.g., HBEC-5i). Here we demonstrate, for the first time, the successful isolation of blood outgrowth endothelial cells (BOECs) from frozen stabilates of peripheral blood mononuclear cells obtained from small-volume peripheral blood samples from paediatric malaria patients. BOECs are a sub-population of human endothelial cells, found within the peripheral blood. We demonstrate that these cells express receptors such as Intercellular Adhesion Molecule 1 (ICAM-1/CD54), Endothelial Protein C Receptor (EPCR/CD201), platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31), Thrombomodulin (CD141), and support adhesion of P. falciparum IEs.
由于缺乏用于研究恶性疟原虫感染红细胞（IEs）细胞黏附的合适动物模型，因此需要进行体外研究，使用各种源自人类肿瘤（例如 BeWo 和 C32 细胞）或非人类来源（例如 CHO 细胞）的细胞系。在现有的人类细胞中，许多细胞源自成年人，或者源自供体池（例如 HBEC-5i）。在这里，我们首次成功地从来自儿科疟疾患者小体积外周血样本的外周血单个核细胞冷冻稳定物中分离出血外生血管内皮细胞（BOECs）。BOECs 是外周血中发现的人类内皮细胞的一个亚群。我们证明这些细胞表达细胞间黏附分子 1（ICAM-1/CD54）、内皮蛋白 C 受体（EPCR/CD201）、血小板/内皮细胞黏附分子 1（PECAM-1/CD31）、血栓调节蛋白（CD141）等受体，并支持恶性疟原虫 IEs 的黏附。
