Laboratory of Ecotoxicology, UMR-I 02 SEBIO, Le Havre University, Normandy University, France; Université de Lorraine, CNRS, LIEC, F-57000 Metz, France.
Laboratory of Ecotoxicology, UMR-I 02 SEBIO, Le Havre University, Normandy University, France.
Mar Pollut Bull. 2018 Oct;135:594-606. doi: 10.1016/j.marpolbul.2018.07.066. Epub 2018 Jul 27.
Intertidal sessile organisms constitute through their life history unintended stress recorders. This study focuses on the impact of pollution on Mytilus edulis ability to cope with an additional stress. For this purpose, two acclimation stages to different temperatures were conducted before an acute stress exposure in mussels collected from a heavily polluted site. Gill proteomes were analyzed by 2DE and regulated proteins identified. Massive mortality was observed for organisms acclimated to colder temperatures. Despite this major difference, both groups shared a common response with a strong representation of proteoforms corresponding to "folding, sorting and degradation" processes. Nevertheless, surviving mussels exhibit a marked increase in protein degradation consistent with the observed decrease of cell defense proteins. Mussels acclimated to warmer temperature response is essentially characterized by an improved heat shock response. These results show the differential ability of mussels to face both pollution and acute heat stress, particularly for low-acclimated organisms.
潮间带固着生物通过其生活史构成了非故意的应激记录器。本研究侧重于污染对贻贝应对额外应激能力的影响。为此，在从污染严重的地点采集的贻贝中进行了两次不同温度的适应阶段，然后进行了急性应激暴露。通过 2DE 分析贻贝的鳃蛋白质组，并鉴定出受调控的蛋白质。适应较低温度的生物体出现了大量死亡。尽管存在这种主要差异，但两组生物体都表现出一种共同的反应，其中强烈代表了与“折叠、分类和降解”过程相对应的蛋白质形式。然而，幸存的贻贝表现出明显的蛋白质降解增加，这与观察到的细胞防御蛋白减少相一致。适应较高温度的贻贝的反应主要以改善的热休克反应为特征。这些结果表明贻贝应对污染和急性热应激的能力存在差异，特别是对于低适应生物体。
