Dupouy J P, Godaut M, Chatelain A
Ann Endocrinol (Paris). 1986;47(6):429-34.
Heparin traps plasma ACTH, promoting the formation of aggregates with apparent high molecular weight as shown by chromatography on Sephadex G 50 fine columns. The percentage of 125I-ACTH which appeared in the void volume of the column, increased linearly with the log. dose of heparin. Heparin at concentrations of up to 100 IU/ml does not impair ACTH adsorption on either silicic acid or Quso G 32 as well as further elution by acetic acid/acetone/water (I : 40 : 59; V/V) or HCl O.I N. Silicic acid traps selectively ACTH but not heparin. Heparin interferes with direct RIA-ACTH in the plasma by decreasing 125I-ACTH binding to the antibodies and modifying the slope of the standard curve. Unsuitable artefacts induced by heparin, as overestimation or underestimation of plasma ACTH levels by RIA, can be avoid by previous hormone extraction from heparinized plasmas. Such results emphasized the importance of the sample preparation in order to obtain consistent results.
肝素能捕获血浆促肾上腺皮质激素（ACTH），促进形成具有明显高分子量的聚集体，这在Sephadex G 50细柱上的色谱分析中得到了证实。出现在柱空体积中的125I - ACTH的百分比随肝素对数剂量呈线性增加。浓度高达100 IU/ml的肝素不会损害ACTH在硅酸或Quso G 32上的吸附，也不会影响随后用乙酸/丙酮/水（1:40:59；V/V）或0.1 N盐酸进行的洗脱。硅酸能选择性地捕获ACTH，但不捕获肝素。肝素通过减少125I - ACTH与抗体的结合并改变标准曲线的斜率，干扰血浆中ACTH的直接放射免疫分析（RIA）。通过预先从肝素化血浆中提取激素，可以避免肝素引起的不适当假象，如RIA对血浆ACTH水平的高估或低估。这些结果强调了样品制备对于获得一致结果的重要性。
