Kaneko S, Murakami S, Kobayashi K, Hattori N
Gan To Kagaku Ryoho. 1987 Mar;14(3 Pt 1):553-60.
Significant research evidence has demonstrated an association between persistent infection with hepatitis B virus (HBV) and the generation of hepatocellular carcinoma (HCC). These findings are based on epidemiologic studies, molecular studies and studies of HBV like viruses. Epidemiologically, the geographic correlation between HBV infection and HCC, serum HBsAg in patients with HCC, familial clustering of HCC, prospective studies, and pathological studies are discussed. Molecular studies of HBV, the structure of HBV DNA as related to retroviruses and integration of HBV DNA into the host DNA are demonstrated. The structure and replication of HBV are somewhat similar to those of retroviruses. The incidence of HBV DNA integration into the host chromosome of the patients with HBV infection is high. The structure of integrated HBV DNA sequences and flanking sequences was analyzed in many cases. However, none of the classical mechanisms of viral oncogenesis has thus far been demonstrated. The role of HBV in HCC is not understood at the molecular level. HBV may act as just an initiator, or HBV DNA integration may have an active role in liver cancer. The woodchuck hepatitis virus (WHV) is the most oncogenic and suitable animal model. Using this model, we show some results of our experiments.
大量研究证据表明，乙型肝炎病毒（HBV）持续感染与肝细胞癌（HCC）的发生之间存在关联。这些发现基于流行病学研究、分子研究以及对乙型肝炎病毒样病毒的研究。在流行病学方面，讨论了HBV感染与HCC之间的地理相关性、HCC患者血清中的HBsAg、HCC的家族聚集性、前瞻性研究以及病理学研究。展示了对HBV的分子研究、与逆转录病毒相关的HBV DNA结构以及HBV DNA整合到宿主DNA中的情况。HBV的结构和复制在某些方面与逆转录病毒相似。HBV感染患者中HBV DNA整合到宿主染色体中的发生率很高。在许多病例中分析了整合后的HBV DNA序列及其侧翼序列的结构。然而，迄今为止尚未证实任何经典的病毒致癌机制。HBV在HCC中的作用在分子水平上尚不清楚。HBV可能仅仅起到启动作用，或者HBV DNA整合可能在肝癌中发挥积极作用。土拨鼠肝炎病毒（WHV）是最具致癌性且合适的动物模型。利用这个模型，我们展示了一些实验结果。
