胰岛素、胰高血糖素、地塞米松、环磷酸鸟苷和精胺对培养的大鼠肝细胞中磷脂酸磷酸水解酶活性稳定性的影响。
Effects of insulin, glucagon, dexamethasone, cyclic GMP and spermine on the stability of phosphatidate phosphohydrolase activity in cultured rat hepatocytes.
作者信息
Pittner R A, Fears R, Brindley D N
出版信息
Biochem J. 1986 Nov 15;240(1):253-7. doi: 10.1042/bj2400253.
Abstract
Hepatocytes were preincubated with 10mM-glucagon and 100 microM-corticosterone to increase phosphatidate phosphohydrolase activity. Addition of 10 nM-glucagon or 100 microM-8-bromo cyclic GMP to a second incubation mixture that contained cycloheximide increased the half-life of the phosphohydrolase activity. Dexamethasone (100 nM) had no significant effect, but insulin (500 pM) or spermine (1 mM) decreased the half-life. None of these compounds altered the general rate of degradation of proteins labelled with [3H]leucine. There appears to be a specific control of the half-life of phosphatidate phosphohydrolase activity, which could contribute to its long-term regulation in the liver.
摘要
将肝细胞与10mM胰高血糖素和100μM皮质酮预孵育，以提高磷脂酸磷酸水解酶活性。向含有环己酰亚胺的第二个孵育混合物中添加10nM胰高血糖素或100μM 8-溴环鸟苷，可增加磷酸水解酶活性的半衰期。地塞米松（100nM）无显著影响，但胰岛素（500pM）或精胺（1mM）可降低半衰期。这些化合物均未改变用[3H]亮氨酸标记的蛋白质的总体降解速率。似乎存在对磷脂酸磷酸水解酶活性半衰期的特异性调控，这可能有助于其在肝脏中的长期调节。
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