Hermier D, Hales P, Brindley D N
Department of Biochemistry, Faculty of Medicine, University of Alberta, Edmonton, Canada.
Biochem J. 1991 Nov 1;279 ( Pt 3)(Pt 3):705-9. doi: 10.1042/bj2790705.
Cultured rat hepatocytes were preincubated with glucagon or a cyclic AMP analogue for up to 24 h and lipid synthesis and secretion were determined during the next 2 h. Glucagon or cyclic AMP did not change the incorporation of choline or glycerol into phosphatidylcholine, or choline into sphingomyelin, in the cells after 0-12 h of preincubation. After 12 h these incorporations were increased. Incorporations into hepatic lysophosphatidylcholine were decreased after preincubation with glucagon or cyclic AMP for 0-12 h, but by 24 h they increased. There was no change in the lysophosphatidylcholine in the medium after preincubation with glucagon or cyclic AMP for up to 6 h, but increases occurred after preincubation from 12 to 24 h. The secretion of triacylglycerol was decreased after preincubation for 0-1 h, but it returned to control values after 4 h. After preincubation for 18-24 h the incorporation of glycerol into secreted triacylglycerol was increased. The results are discussed in relation to the control of lipid metabolism in starvation and diabetes.
将培养的大鼠肝细胞与胰高血糖素或环磷酸腺苷类似物预孵育长达24小时，然后在接下来的2小时内测定脂质合成和分泌情况。在预孵育0 - 12小时后，胰高血糖素或环磷酸腺苷不会改变胆碱或甘油掺入磷脂酰胆碱，或胆碱掺入鞘磷脂的情况。预孵育12小时后，这些掺入量增加。在与胰高血糖素或环磷酸腺苷预孵育0 - 12小时后，肝溶血磷脂酰胆碱的掺入量减少，但到24小时时增加。与胰高血糖素或环磷酸腺苷预孵育长达6小时后，培养基中的溶血磷脂酰胆碱没有变化，但预孵育12至24小时后增加。预孵育0 - 1小时后三酰甘油的分泌减少，但4小时后恢复到对照值。预孵育18 - 24小时后，甘油掺入分泌的三酰甘油中的量增加。结合饥饿和糖尿病状态下脂质代谢的调控对结果进行了讨论。
