超氧化物介导的核苷酸碱基释放及磷酸基团的分子内增强效应。

Base liberation from nucleotides by superoxide and intramolecular enhancement effect of phosphate group.

作者信息

Yamane H, Yada N, Katori E, Mashino T, Nagano T, Hirobe M

出版信息

Biochem Biophys Res Commun. 1987 Feb 13;142(3):1104-10. doi: 10.1016/0006-291x(87)91529-4.

Abstract

The reaction of nucleotides with superoxide gave the corresponding nucleobases in good yield. The base-release reaction of nucleotides due to superoxide was examined and compared to that of nucleosides. Hence, the phosphate moiety greatly enhances the yields, in particular those of adenine from adenosine monophosphates. Superoxide in combination with the phosphate moiety has been revealed to be more active than superoxide alone. The phosphate peroxy radical, generated in situ from superoxide and phosphate, seems to be the species responsible for the formation of the free bases.

摘要

核苷酸与超氧化物反应能以良好的产率生成相应的核碱基。研究了超氧化物导致的核苷酸碱基释放反应，并与核苷的该反应进行了比较。因此，磷酸部分极大地提高了产率，尤其是一磷酸腺苷生成腺嘌呤的产率。已发现超氧化物与磷酸部分结合比单独的超氧化物更具活性。由超氧化物和磷酸盐原位生成的过氧磷酸根自由基似乎是负责游离碱基形成的物质。