Ho A K, Ling Q L, Duffield R, Lam P H, Wang J H
Biochem Biophys Res Commun. 1987 Feb 13;142(3):911-8. doi: 10.1016/0006-291x(87)91500-2.
Muscarinic receptor, from porcine synaptic membrane, was purified by affinity chromatography. Molecular weight analysis by SDS-gel electrophoresis revealed one major peptide with an apparent Mr of 68 +/- 2 Kda. The purified receptor was phosphorylated by the catalytic subunit of cAMP-dependent protein kinase resulting in a concomitant loss in specific binding, and this loss was reversed by calcineurin.
