Solito Samantha, Pinton Laura, De Sanctis Francesco, Ugel Stefano, Bronte Vincenzo, Mandruzzato Susanna, Marigo Ilaria
Oncology and Immunology Section, Department of Surgery, Oncology and Gastroenterology, University of Padova, Padova, Italy.
Veneto Institute of Oncology IOV- IRCCS, Padova, Italy.
Curr Protoc Immunol. 2019 Feb;124(1):e61. doi: 10.1002/cpim.61. Epub 2018 Oct 10.
This unit presents methods to assess the immunosuppressive properties of immunoregulatory cells of myeloid origin, such as myeloid-derived suppressor cells (MDSCs), both in vitro and in vivo in mice, as well as in biological samples from cancer patients. These methods could be adapted to test the impact of different suppressive populations on T cell activation, proliferation, and cytotoxic activity; moreover, they could be useful to assess the influence exerted by genetic modifications, chemical inhibitors, and drugs on immune suppressive pathways © 2018 by John Wiley & Sons, Inc.
本单元介绍了评估髓系来源的免疫调节细胞（如髓系来源的抑制细胞 (MDSC)）免疫抑制特性的方法，这些方法可用于在小鼠体内外以及癌症患者的生物样本中进行评估。这些方法可用于测试不同抑制性细胞群体对 T 细胞活化、增殖和细胞毒性活性的影响；此外，它们还可用于评估基因修饰、化学抑制剂和药物对免疫抑制途径的影响。© 2018 约翰威立国际出版公司。
