Department of Applied and Ecological Microbiology, Institute of Microbiology, Friedrich Schiller University Jena, Philosophenweg 12, 07743 Jena, Germany.
FEMS Microbiol Ecol. 2019 Jan 1;95(1). doi: 10.1093/femsec/fiy199.
Sulfuritalea hydrogenivorans sk43H is well recognized as a chemolithoautotrophic microorganism that oxidizes thiosulfate, sulfur or hydrogen. In this study, pathways for aromatic compound degradation were identified in the respective genome and proved for functionality by cultivation. S. hydrogenivorans sk43H harbors gene clusters encoding pathways for the anaerobic degradation of benzoate and phenylacetate via benzoyl-CoA as well as a partial pathway for anaerobic cinnamate degradation. Aerobic hybrid pathways were identified for the degradation of benzoate and 2-aminobenzoate. An aerobic pathway involving mono- and dioxygenases was found for 4-hydroxybenzoate. The organization of the gene clusters for anaerobic aromatic compound degradation in S. hydrogenivorans sk43H was found to be similar to that of the corresponding gene clusters in 'Aromatoleum aromaticum' strain EbN1. Cultivation experiments revealed that S. hydrogenivorans sk43H degrades benzoate, 4-hydroxybenzoate, phenylacetate and 4-hydroxyphenylacetate under nitrate-reducing conditions. The results imply a so far overlooked role of this microorganism in anaerobic aromatic compound degradation. Due to the frequent detection of Sulfuritalea-related microorganisms at hydrocarbon-contaminated sites, an involvement of this genus in the degradation of aromatic pollutants should be considered.
硫矿硫化叶菌 sk43H 是一种众所周知的化能自养微生物，能够氧化硫代硫酸盐、硫或氢。在这项研究中，在相应的基因组中确定了芳香族化合物降解途径，并通过培养证明了其功能。S. hydrogenivorans sk43H 拥有编码苯甲酰辅酶 A 途径的基因簇，用于厌氧降解苯甲酸和苯乙酸，以及用于厌氧肉桂酸盐降解的部分途径。鉴定出了用于降解苯甲酸和 2-氨基苯甲酸的好氧混合途径。发现了一种涉及单加氧酶和双加氧酶的 4-羟基苯甲酸好氧途径。在 S. hydrogenivorans sk43H 中，厌氧芳香族化合物降解的基因簇的组织类似于相应的基因簇在“Aromatoleum aromaticum”菌株 EbN1 中的组织。培养实验表明，S. hydrogenivorans sk43H 在硝酸盐还原条件下可以降解苯甲酸、4-羟基苯甲酸、苯乙酸和 4-羟基苯乙酸。这些结果暗示了该微生物在厌氧芳香族化合物降解中被忽视的作用。由于在烃污染场所经常检测到硫矿硫化叶菌相关微生物，因此应该考虑该属参与芳香族污染物的降解。
