[α-生育酚与游离脂肪酸的相互作用。脂质双层微粘度稳定机制]

[Interaction of alpha-tocopherol with free fatty acids. Mechanism of stabilization of lipid bilayer microviscosity].

作者信息

Erin A N, Gorbunov N V, Skrypin V I, Kagan V E, Prilipko L L

出版信息

Nauchnye Doki Vyss Shkoly Biol Nauki. 1987(1):10-6.

Abstract

Using ESR-spin probes and 1H-NMR-spectroscopy methods the effect of alpha-tocopherol on liposome microviscosity has been studied. alpha-Tocopherol has been shown to remove the chaotropic action of free fatty acids on bilayer. The stabilization effect found has a common nature and does not depend on the chemical structure of the phopsholipid functional polar groups, the unsaturation degree of free fatty acids as well as fatty acids residua entering into phospholipid composition. Analog of alpha-tocopherol without phytol chain 2,2,5,7,8-penthamethyl-6-oxychroman does not show the stabilizing effect on the microviscosity of lipid bilayer under the action of free fatty acids. It indicates that both chromanol nucleus and phytol chain of alpha-tocopherol molecule are necessary for stabilizing action. The data obtained allow to suppose that the interaction of alpha-tocopherol with free fatty acids may be one of the molecular mechanisms of lipid bilayer microvicosity stabilization.

摘要

运用电子自旋共振（ESR）自旋探针和核磁共振氢谱（1H-NMR）光谱法，研究了α-生育酚对脂质体微粘度的影响。结果表明，α-生育酚可消除游离脂肪酸对双层膜的离液作用。所发现的稳定作用具有共性，且不依赖于磷脂功能极性基团的化学结构、游离脂肪酸的不饱和度以及进入磷脂组成的脂肪酸残基。不含叶绿醇链的α-生育酚类似物2,2,5,7,8-五甲基-6-氧代色满在游离脂肪酸作用下，对脂质双层的微粘度未表现出稳定作用。这表明α-生育酚分子的色满醇核和叶绿醇链对于稳定作用都是必需的。所得数据表明，α-生育酚与游离脂肪酸的相互作用可能是脂质双层微粘度稳定的分子机制之一。