Department of Chemistry, University of Kurdistan, 66177-15175, Sanandaj, Iran.
Research Centre for Nanotechnology, University of Kurdistan, 66177-15175, Sanandaj, Iran.
Sci Rep. 2018 Oct 10;8(1):15103. doi: 10.1038/s41598-018-32893-2.
Bipolar electrochemistry (BPE) has been lately explored as a simple, reliable and novel electrochemical technique for the adjustment of various conductive substrates. Herein, BPE is performed to derive both of cathode and anode electrodes for the development of mediatorless/membraneless biofuel cell (BFC). On one hand, a preferable substrate for immobilization of bilirubin oxidase enzyme is prepared based on the electropolymerization of thiophene-3-carboxcylic acid (TCA) on an Au microfilm as a bipolar electrode. The resulted biocathode as novel bioelectrocatalyst offers a high electrocatalytic activity toward direct oxygen reduction reaction (ORR) with onset potential and current density of 0.55 V (vs. Ag/AgCl) and 867 μA cm, respectively. On the other hand, another analogous Au bipolar electrode is electroplated through BPE to derive Au nanostructures (AuNSs). This modified Au electrode is utilized as an anodic platform for immobilization of flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH) enzyme aimed at electrocatalytic glucose oxidation. The prepared bioanode displays a current density of 2.7 mA cm with onset potential of -0.03 V. Finally, the proposed bioanode and biocacthode in an assembled membraneless glucose/O BFC offers a power output of 146 μW cm with open circuit voltage of 0.54 V. This novel BPE method provides disposable electrochemical platforms for design of novel sensors, biosensors or other devices.
双极电化学（BPE）最近被探索作为一种简单、可靠和新颖的电化学技术，用于调整各种导电基底。本文采用 BPE 来衍生阴极和阳极电极，用于开发无介体/无膜生物燃料电池（BFC）。一方面，基于噻吩-3-羧酸（TCA）在 Au 微膜上电聚合，制备了一种更好的胆红素氧化酶固定基底，作为双极电极。所得的生物阴极作为新型生物电化学催化剂，对直接氧还原反应（ORR）表现出高电催化活性，起始电位和电流密度分别为 0.55 V（相对于 Ag/AgCl）和 867 μA cm。另一方面，通过 BPE 电沉积另一个类似的 Au 双极电极，衍生出 Au 纳米结构（AuNSs）。修饰后的 Au 电极用作固定黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶（FAD-GDH）酶的阳极平台，用于电催化葡萄糖氧化。所制备的生物阳极的电流密度为 2.7 mA cm，起始电位为-0.03 V。最后，在组装的无膜葡萄糖/O BFC 中，提出的生物阳极和生物阴极提供了 146 μW cm 的功率输出，开路电压为 0.54 V。这种新型 BPE 方法为设计新型传感器、生物传感器或其他设备提供了一次性电化学平台。
