Kaysen J H, Amari N M, Lambert M W
Cell Biol Int Rep. 1987 Feb;11(2):95-101. doi: 10.1016/0309-1651(87)90109-3.
Positioning of nucleosomes was examined in a reconstituted system using a plasmid DNA and histones from normal human and xeroderma pigmentosum, complementation group A (XPA), lymphoblastoid cells. The present studies indicate that the arrangement of nucleosomes, composed of normal human histones, in a region near the SV40 origin of replication on the plasmid DNA, is nonrandom. The alignment of nucleosomes in this region was not affected by the presence of histone H1. No difference in nucleosome positioning was observed when the nucleosomes were composed of histones from XPA cells.
使用来自正常人及着色性干皮病A组（XPA）淋巴母细胞样细胞的质粒DNA和组蛋白，在一个重组系统中检测核小体的定位。目前的研究表明，由正常人组蛋白组成的核小体在质粒DNA上SV40复制起点附近区域的排列是非随机的。该区域核小体的排列不受组蛋白H1存在的影响。当核小体由XPA细胞的组蛋白组成时，未观察到核小体定位有差异。
