Zakaria Muhammad Azman, Mohd Yusoff Mohd Zulkhairi, Zakaria Mohd Rafein, Hassan Mohd Ali, Wood Thomas K, Maeda Toshinari
1Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia.
2Laboratory of Biopolymer and Derivatives, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Malaysia.
3 Biotech. 2018 Oct;8(10):435. doi: 10.1007/s13205-018-1461-2. Epub 2018 Oct 3.
Pseudogenes in the genome are assumed to be non-functional. In this study, Keio collection BW25113∆ and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant decreased biohydrogen production from 37 µmol mg protein to 6 µmol mg protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the mutation represses transcription tenfold; encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of . Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in .
基因组中的假基因被认为是无功能的。在本研究中，使用了Keio菌株BW25113∆和产YqiG的菌株（BW25113/pCA24N-YqiG）来评估假基因在氢代谢中的重要性。我们的结果表明，假基因蛋白YqiG被鉴定为从葡萄糖生产生物氢过程中的一种必需蛋白。与野生型菌株相比，该突变体的生物氢产量从37微摩尔/毫克蛋白降至6微摩尔/毫克蛋白，葡萄糖消耗量减少了80%。通过转录分析，我们发现该突变使转录受到十倍的抑制；YqiG编码丙酮酸甲酸裂解酶，它是厌氧代谢中的关键酶之一。此外，与野生型菌株相比，YqiG的产生刺激了糖酵解并使生物氢生产力提高了1.5倍。因此，YqiG对于中心糖酵解反应很重要，并且能够影响生物体内的氢代谢活性。
