Pseudogene product YqiG is important for expression and biohydrogen production in BW25113.

Pseudogenes in the genome are assumed to be non-functional. In this study, Keio collection BW25113∆ and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant decreased biohydrogen production from 37 µmol mg protein to 6 µmol mg protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the mutation represses transcription tenfold; encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of . Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in .