Kesici Gülin Gökçen, Öcal Fatma Ceyda Akın, Gürgen Seren Gülşen, Erdem Şaban Remzi, Öğüş Ersin, Erbek Hatice Seyra, Özlüoğlu Levent Naci
Department of ENT, Ankara Atatürk Education and Research Hospital, 06800, Ankara, Turkey.
Department of ENT, Gülhane Education and Research Hospital, 06010, Ankara, Turkey.
Eur Arch Otorhinolaryngol. 2018 Dec;275(12):2957-2966. doi: 10.1007/s00405-018-5161-7. Epub 2018 Oct 10.
To test the protective effect of metformin against noise-induced hearing loss.
24 rats were included in the study. The first group was exposed to noise only, the second group took metformin, the third group was exposed to noise and took metformin, and the fourth group was neither exposed to noise nor took metformin as control group. After measurement of baseline DPOAE and ABR of rats, the metformin group and the metformin + noise group received 300 mg/kg/day metformin via gavage for 10 days. On the 11th day, group 1 and group 3 were exposured to white noise at 105 dB SPL for 15 h. After noise exposure, DPOAE and ABR measurements of all rats were repeated on days 1st, 7th, and 21st. At the end of the study, all animals were sacrificed and cochlear tissues were separated for immunohistochemical assessments.
ABR threshold values and DPAOE measurements of groups 1 and 3 were deteriorated on the 1st day after noise, while deterioration in group 1 continued on 7th and 21st days, but normalized on 7th day in group 3. After immune staining, a significant immunoreaction was observed in the noise group, while the reaction in the noise + metformin group was close to the control group.
Metformin has a protective effect on noise-induced hearing loss in rats. As a conclusion, it is determined that metformin protects from permanent threshold shift in rats. It can be considered a good alternative for protecting noise-induced hearing loss.
测试二甲双胍对噪声性听力损失的保护作用。
24只大鼠纳入研究。第一组仅暴露于噪声，第二组服用二甲双胍，第三组暴露于噪声并服用二甲双胍，第四组既不暴露于噪声也不服用二甲双胍作为对照组。在测量大鼠的基线畸变产物耳声发射（DPOAE）和听性脑干反应（ABR）后，二甲双胍组和二甲双胍+噪声组经口灌胃给予300 mg/kg/天的二甲双胍，持续10天。在第11天，第1组和第3组暴露于105 dB SPL的白噪声中15小时。噪声暴露后，在第1、7和21天重复测量所有大鼠的DPOAE和ABR。在研究结束时，处死所有动物并分离耳蜗组织进行免疫组织化学评估。
第1组和第3组在噪声暴露后第1天的ABR阈值和DPOAE测量值恶化，而第1组在第7天和第21天持续恶化，但第3组在第7天恢复正常。免疫染色后，噪声组观察到明显的免疫反应，而噪声+二甲双胍组的反应接近对照组。
二甲双胍对大鼠噪声性听力损失具有保护作用。总之，确定二甲双胍可保护大鼠免受永久性阈移。它可被认为是保护噪声性听力损失的一个良好选择。
