Lima Debora B, Rezende-Puker Daniela, Mendonça Renata S, Tixier Marie-Stephane, Gondim Manoel G C, Melo José W S, Oliveira Daniel C, Navia Denise
Departamento de Agronomia - Entomologia, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, Dois Irmãos, Recife, PE, 52171-900, Brazil.
Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Campus Darcy Ribeiro, ICC Centro, Asa Norte, Brasília, DF, 70297-400, Brazil.
Exp Appl Acarol. 2018 Nov;76(3):287-310. doi: 10.1007/s10493-018-0308-1. Epub 2018 Oct 10.
The accurate characterization of biological control agents is a key step in control programs. Recently, Amblyseius largoensis from Thailand were introduced in Brazil to evaluate their efficiency for the control of the red palm mite, Raoiella indica. The aim of this study was to confirm their identification and to characterize the population from Thailand, comparing it to populations of the Americas and Indian Ocean islands. In addition, a population of A. largoensis from New Caledonia, Oceania, of which DNA sequences were available, was included in phylogenetic analyses. Morphometric data obtained for the population of A. largoensis from Thailand were compared to those of populations from Reunion Island and the Americas through univariate and multivariate analyses. Two DNA fragments were amplified and sequenced: the nuclear ribosomal region ITSS and the mitochondrial 12S rRNA. Haplotypes (12S rRNA) and genotypes (ITSS) were identified and phylogenetic analyses using both fragments were conducted separately and combined using maximum likelihood and the Bayesian information criterion. The integrative approach reveals morphometric and molecular variabilities among populations of A. largoensis and shows that the population identified as A. largoensis collected in Thailand, as well as that from New Caledonia, are conspecific to the populations of the Americas and Indian Ocean islands. Populations from the Americas and Asia are more related to each other than with that from the Indian Ocean islands. Hypotheses to explain this clustering are proposed. Data on the molecular intraspecific variability of this predatory mite from remote areas will be helpful for the development of molecular diagnosis.
生物防治剂的准确鉴定是防治计划中的关键一步。最近，来自泰国的拉哥钝绥螨被引入巴西，以评估其对红棕叶螨的防治效果。本研究的目的是确认其身份，并对来自泰国的种群进行特征描述，将其与美洲和印度洋岛屿的种群进行比较。此外，来自大洋洲新喀里多尼亚的拉哥钝绥螨种群（已有其DNA序列）被纳入系统发育分析。通过单变量和多变量分析，将从泰国获得的拉哥钝绥螨种群的形态测量数据与留尼汪岛和美洲种群的数据进行比较。扩增并测序了两个DNA片段：核糖体ITS2区域和线粒体12S rRNA。鉴定了单倍型（12S rRNA）和基因型（ITS2），并分别使用最大似然法和贝叶斯信息准则对两个片段进行系统发育分析，然后将结果合并。综合分析方法揭示了拉哥钝绥螨种群间的形态和分子变异，并表明在泰国采集的被鉴定为拉哥钝绥螨的种群以及来自新喀里多尼亚的种群与美洲和印度洋岛屿的种群是同种的。美洲和亚洲的种群彼此之间的关系比与印度洋岛屿的种群更为密切。文中提出了解释这种聚类现象的假设。来自偏远地区的这种捕食螨的分子种内变异数据将有助于分子诊断技术的发展。
