Tullo A B, Coupes D, Klapper P, Cleator G, Chitkara D
Curr Eye Res. 1987 Jan;6(1):33-8. doi: 10.3109/02713688709020065.
An in vitro analysis of glycoprotein produced by nine human ocular isolates of HSV-1 is reported. The source of the isolates was; three patients with recurrent dendritic keratitis, three with chronic stromal disease and three with primary keratoconjunctivitis. Virus strains were labelled with the radioactive precursors (35S) methionine and (14C) glucosamine. Radiolabelled viral glycoproteins were subsequently analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), followed by autoradiography. Viral glycoproteins were further characterised by immuno-precipitation with polyclonal and monoclonal antibodies to HSV. The stromal isolates excrete larger amounts of 'soluble' precursor glycoprotein D than those in the other two disease categories. It is possible that the immune response to glycoprotein D is in part responsible for the severity of stromal disease.
本文报道了对9株人单纯疱疹病毒1型（HSV-1）眼部分离株所产生糖蛋白的体外分析。这些分离株的来源如下：3例复发性树枝状角膜炎患者、3例慢性基质疾病患者和3例原发性角膜结膜炎患者。病毒株用放射性前体（35S）甲硫氨酸和（14C）葡糖胺进行标记。随后，通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）分析放射性标记的病毒糖蛋白，接着进行放射自显影。通过用针对HSV的多克隆和单克隆抗体进行免疫沉淀，对病毒糖蛋白作进一步表征。基质分离株比其他两类疾病的分离株分泌更多量的“可溶性”前体糖蛋白D。对糖蛋白D的免疫反应可能在一定程度上导致了基质疾病的严重程度。
