Moura I, Fauque G, LeGall J, Xavier A V, Moura J J
Eur J Biochem. 1987 Feb 2;162(3):547-54. doi: 10.1111/j.1432-1033.1987.tb10674.x.
Two c-type cytochromes were purified and characterized by electron paramagnetic resonance (EPR) and nuclear magnetic resonance (NMR) spectroscopic techniques, from the sulfate-reducer nitrogen-fixing organism, Desulfovibrio desulfuricans strain Berre-Eau (NCIB 8387). The purification procedures included several chromatographic steps on alumina, carboxymethylcellulose and gel filtration. A tetrahaem and a monohaem cytochrome were identified. The multihaem cytochrome has visible, EPR and NMR spectra with general properties similar to other low-potential bis-histidinyl axially bound haem proteins, belonging to the class of tetrahaem cytochrome c3 isolated from other Desulfovibrio species. The monohaem cytochrome c553 is ascorbate-reducible and its EPR and NMR data are characteristic of a cytochrome with methionine-histidine ligation. Their properties are compared with other homologous proteins isolated from sulfate-reducing bacteria.
从硫酸盐还原固氮微生物脱硫脱硫弧菌贝雷 - 欧菌株（NCIB 8387）中纯化出两种c型细胞色素，并通过电子顺磁共振（EPR）和核磁共振（NMR）光谱技术对其进行了表征。纯化程序包括在氧化铝、羧甲基纤维素上的几个色谱步骤以及凝胶过滤。鉴定出一种四血红素细胞色素和一种单血红素细胞色素。多血红素细胞色素具有可见光谱、EPR光谱和NMR光谱，其一般性质与其他低电位双组氨酸轴向结合血红素蛋白相似，属于从其他脱硫弧菌属物种中分离出的四血红素细胞色素c3类别。单血红素细胞色素c553可被抗坏血酸还原，其EPR和NMR数据是具有甲硫氨酸 - 组氨酸连接的细胞色素的特征。将它们的性质与从硫酸盐还原细菌中分离出的其他同源蛋白进行了比较。
