Fares F, Bar-Ami S, Brandes J M, Gavish M
Eur J Pharmacol. 1987 Jan 6;133(1):97-102. doi: 10.1016/0014-2999(87)90210-x.
Peripheral-type benzodiazepine binding sites (PBS) were demonstrated in the cell membranes of various organs (ovary, uterus, oviduct, pituitary and kidney) of mature and immature female rats by using the PBS-specific ligand [3H]PK 11195. The equilibrium dissociation constants of [3H]PK 11195 for PBS in mature rats ranged from 3 to 4 nM. The specific binding of [3H]PK 11195 (2 nM) in the hypophyseal-genital axis of immature (19-27 days old) female rats was found to be significantly increased in the ovary and uterus, concurrently with the increase in age. Administration of pregnant mare serum gonadotropin or diethylstilbestrol to immature rats increased the density of PBS in the ovary and uterus 2- to 3-fold but no change was found in the kidney. The affinity of [3H]PK 11195 to these tissues did not change following hormonal treatment. These results suggest that gonadotropin and estrogen are involved in the induction of PBS in the organs of the hypophyseal-genital axis in female rats.
利用外周型苯二氮䓬结合位点（PBS）特异性配体[3H]PK 11195，在成熟和未成熟雌性大鼠的各种器官（卵巢、子宫、输卵管、垂体和肾脏）细胞膜中证实了外周型苯二氮䓬结合位点（PBS）。成熟大鼠中[3H]PK 11195与PBS的平衡解离常数范围为3至4 nM。发现未成熟（19 - 27日龄）雌性大鼠垂体 - 生殖轴中[3H]PK 11195（2 nM）的特异性结合在卵巢和子宫中随年龄增长而显著增加。给未成熟大鼠注射孕马血清促性腺激素或己烯雌酚可使卵巢和子宫中PBS的密度增加2至3倍，但肾脏中未发现变化。激素处理后，[3H]PK 11195与这些组织的亲和力未改变。这些结果表明，促性腺激素和雌激素参与了雌性大鼠垂体 - 生殖轴器官中PBS的诱导。
