Fares F, Bar-Ami S, Haj-Yehia Y, Gavish M
Department of Pharmacology, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa.
J Recept Res. 1989;9(2):143-57. doi: 10.3109/10799898909066050.
The effect of hypophysectomy and hormonal replacement on the density of peripheral benzodiazepine binding sites (PBS) in rat adrenal gland and kidney was studied. In the adrenal gland, hypophysectomy caused a significant decrease of 3-fold in PBS density. In the kidney, in contrast, hypophysectomy did not affect PBS density. In the adrenal gland, adrenocorticotropic hormone (ACTH) administered to hypophysectomized rats caused a significant increase of more than 5-fold in PBS density compared to untreated hypophysectomized rats, and of more than 1.6-fold compared to intact rats. In contrast, the hormones pregnant mare serum gonadotropin (PMSG), diethylstilbestrol (DES), and hydrocortisone (HC), administered to hypophysectomized rats, failed to restore PBS density in the adrenal gland. In the kidney, HC administered to hypophysectomized rats caused an increase of 1.4-fold in PBS density compared to untreated hypophysectomized and intact rats. In contrast, the hormones ACTH, PMSG, and DES, administered to hypophysectomized rats, did not affect PBS density in the kidney. None of the hormones tested altered the equilibrium dissociation constant of PBS in either the adrenal gland or the kidney. These findings indicate that PBS density in rat adrenal gland and kidney is hormonally modulated.
研究了垂体切除及激素替代对大鼠肾上腺和肾脏外周苯二氮䓬结合位点（PBS）密度的影响。在肾上腺中，垂体切除导致PBS密度显著下降3倍。相比之下，在肾脏中，垂体切除并未影响PBS密度。在肾上腺中，给垂体切除的大鼠注射促肾上腺皮质激素（ACTH），与未处理的垂体切除大鼠相比，PBS密度显著增加超过5倍，与完整大鼠相比增加超过1.6倍。相反，给垂体切除的大鼠注射孕马血清促性腺激素（PMSG）、己烯雌酚（DES）和氢化可的松（HC），未能恢复肾上腺中的PBS密度。在肾脏中，与未处理的垂体切除大鼠和完整大鼠相比，给垂体切除的大鼠注射HC导致PBS密度增加1.4倍。相反，给垂体切除的大鼠注射ACTH、PMSG和DES，并未影响肾脏中的PBS密度。所测试的激素均未改变肾上腺或肾脏中PBS的平衡解离常数。这些发现表明，大鼠肾上腺和肾脏中的PBS密度受激素调节。
