Department of Ophthalmology, The Eye Center of the First Affiliated Hospital of Fujian Medical University, Fujian Eye Institute , Fu Zhou , P.R. , China.
Ocul Immunol Inflamm. 2019;27(6):958-967. doi: 10.1080/09273948.2018.1491604. Epub 2018 Oct 11.
: To investigate the immune response and mechanisms of interferon-γ (IFN-γ) in the fungal keratitis in mice. : Mice were divided into two groups: group A, topical PBS four times daily post-infection; group B: topical IFN-γ four times daily post-infection. At1, 3, 5, and 7 days, the corneal lesions and inflammatory responses were observed by slit lamp, and immunofluorescence staining was performed to evaluate F4/80+ and CD4+ cells. Using ELISA, and RT-PCR to detect the expression levels of macrophage migration inhibitory factor (MIF), macrophage inflammatory protein-2 (MIP-2), IL-4, IL-10, IL-12, and IFN-γ. : The treatment with IFN-γ decreased clinical scores and expression levels of IL-4, increased expression of F4/80+ and CD4+ cells, whereas IL-12, MIF, and MIP-2 were expressed highly, and the peaks of IL-10 and IFN-γ move forward. : This experiment showed that IFN-γ eye drops increase the accumulation of macrophages and shorten the duration of fungal keratitis.
: 目的：研究干扰素-γ（IFN-γ）在小鼠真菌性角膜炎中的免疫反应和作用机制。 : 方法：将小鼠分为两组：A 组，感染后每日用 PBS 滴眼 4 次；B 组，感染后每日用 IFN-γ 滴眼 4 次。在 1、3、5、7 天，用裂隙灯观察角膜病变和炎症反应，并用免疫荧光染色法评估 F4/80+和 CD4+细胞。用 ELISA 和 RT-PCR 检测巨噬细胞移动抑制因子（MIF）、巨噬细胞炎症蛋白-2（MIP-2）、IL-4、IL-10、IL-12 和 IFN-γ的表达水平。 : IFN-γ 滴眼治疗降低了临床评分和 IL-4 的表达水平，增加了 F4/80+和 CD4+细胞的表达水平，而 IL-12、MIF 和 MIP-2 的表达水平较高，IL-10 和 IFN-γ 的表达峰值提前。 : 本实验表明，IFN-γ 滴眼剂增加了巨噬细胞的聚集，缩短了真菌性角膜炎的持续时间。
