Hammonds P, Schofield P N, Ashcroft S J
FEBS Lett. 1987 Mar 9;213(1):149-54. doi: 10.1016/0014-5793(87)81481-3.
In HIT-T15 cells grown in the absence of glucose, Northern blot analysis of total RNA revealed a major 0.5 kb preproinsulin (ppI) mRNA transcript which co-migrated with the mature transcript from a human insulinoma. In 4 h tissue cultures, glucose (2-20 mM) stimulated HIT cell ppI mRNA levels in a markedly dose-dependent manner. Glucose-stimulated ppI mRNA was (i) inhibited by actinomycin D, suggesting that regulation may be in part transcriptional, and (ii) potentiated by agents known to activate B cell protein kinases. HIT cells represent a unique model for investigating long term regulation of insulin gene expression and biosynthesis.
在无葡萄糖条件下培养的HIT-T15细胞中，对总RNA进行Northern印迹分析发现，有一个主要的0.5 kb前胰岛素原（ppI）mRNA转录本，其迁移率与人胰岛素瘤的成熟转录本相同。在4小时的组织培养中，葡萄糖（2 - 20 mM）以明显的剂量依赖性方式刺激HIT细胞的ppI mRNA水平。葡萄糖刺激的ppI mRNA：（i）被放线菌素D抑制，这表明调节可能部分是转录水平的；（ii）被已知可激活B细胞蛋白激酶的试剂增强。HIT细胞是研究胰岛素基因表达和生物合成长期调节的独特模型。
