Yonuschot G, Matthews E K, Corps A N, Metcalfe J C
FEBS Lett. 1987 Mar 23;213(2):401-5. doi: 10.1016/0014-5793(87)81530-2.
Thymocytes previously loaded with quin 2 were rapidly permeabilized by the photon activation of erythrosin and the rate of permeabilization monitored by measuring fluorimetrically the increasing saturation of quin 2 with calcium. The extent of permeabilization was assessed also by the loss of [3H]quin 2 from the thymocytes and penetration of the cells by eosin and trypan blue. Lactate dehydrogenase leakage from the permeabilized cells was markedly delayed compared to the rapid increase in permeability to calcium and quin 2. The rate of permeabilization was dependent upon the concentration of erythrosin, the duration of illumination, the presence of oxygen, and the temperature. These results are consistent with the rapid photochemical generation of highly reactive singlet oxygen which alters thymocyte membrane structure and permeability.
预先加载了喹啉-2的胸腺细胞通过赤藓红的光子激活迅速被通透化，通过荧光测量喹啉-2与钙的饱和度增加来监测通透化速率。通透化程度还通过胸腺细胞中[3H]喹啉-2的损失以及伊红和台盼蓝对细胞的穿透来评估。与钙和喹啉-2通透性的快速增加相比，通透化细胞中乳酸脱氢酶的泄漏明显延迟。通透化速率取决于赤藓红的浓度、光照持续时间、氧气的存在以及温度。这些结果与高活性单线态氧的快速光化学产生一致，后者改变了胸腺细胞膜结构和通透性。
