Head of Department of biomedical Engineering, Military Technical Collage, Kobry Elkoba, Cairo, Egypt.
Head of Nanotechnology Research Center, Military Technical College, Kobry El-Kobba, Cairo, Egypt.
Photodiagnosis Photodyn Ther. 2018 Dec;24:166-178. doi: 10.1016/j.pdpdt.2018.10.004. Epub 2018 Oct 9.
Early detection of carious is vital for demineralization reversal, offering less pain, as well as precise carious removal. In this study, the difference in optical properties of normal tissue and human carious lesion has been used for early diagnosis, using laser induced fluorescence spectroscopy. The optical system consists of light source in visible band and hyperspectral camera, associated with designed digital image processing algorithm. The human tooth sample was illuminated with visible band sources at 488, and 514 nm with energy of 5 m watt. The reflected and emitted light from the tested sample was captured using hyperspectral camera in an attempt to generate multispectral images (cubic image). The variation of reflected and emitted energy as function of wavelength was employed to generate characteristic spectrum of each tooth tissue. Human teeth carious tissue lesion releases its excess energy by emitting fluorescence light producing chemical footprint signature; this signature is dependent on the elemental composition of tooth elements and carious state. This non-invasive, non-contact and non-ionizing imaging system with associated novel pattern recognition algorithm was employed to diagnose and classify different carious types and stages. It was reported that the perceived fluorescence emission is function of the illuminating wavelength. While enamel and dentin carious were distinguished and characterized at 514 nm illuminating wavelength; white spot lesion were contoured and recognized at 488 nm. Therefore, full recognition could be achieved through generated cubic image after sample irradiation at 488 nm and 514 nm. In conclusion, this study reports on a customized optical image system that can offer high sensitivity, high resolution, and early carious detection with optimum performance at 514 nm and 488 nm.
早期发现龋齿对于脱矿逆转至关重要,不仅可以减轻疼痛,还能更精确地去除龋齿。在这项研究中,利用激光诱导荧光光谱学,通过正常组织和人龋齿病变的光学特性差异进行早期诊断。光学系统由可见波段光源和高光谱相机组成,并结合设计的数字图像处理算法。用 488nm 和 514nm 的可见波段光源对人牙样本进行照射,能量为 5mW。用高光谱相机捕获来自测试样本的反射和发射光,试图生成多光谱图像(立方图像)。反射和发射能量随波长的变化被用来生成每个牙齿组织的特征光谱。人牙龋齿病变通过发射荧光释放多余能量,产生化学特征签名;该签名取决于牙齿元素的元素组成和龋齿状态。这种非侵入式、非接触式和非电离成像系统以及相关的新型模式识别算法被用于诊断和分类不同类型和阶段的龋齿。据报道,感知到的荧光发射是与照射波长有关的函数。在 514nm 照射波长下可以区分和表征釉质和牙本质龋齿,而在 488nm 下可以勾勒和识别白点病变。因此,通过在 488nm 和 514nm 照射后生成的立方图像可以实现全面识别。总之,本研究报告了一种定制的光学成像系统,该系统在 514nm 和 488nm 下具有高灵敏度、高分辨率和早期龋齿检测的最佳性能。