El-Sharkawy Yasser H, Elbasuney Sherif
Department of Biomedical Engineering, Military Technical College, Kobry Elkoba, Cairo, Egypt.
Nanotechnology Center, Military Technical College, Kobry El-Kobba, Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 15;240:118616. doi: 10.1016/j.saa.2020.118616. Epub 2020 Jun 19.
There is a must for non-invasive caries detection method to supplement traditional visual assessment by the clinician before lesion progression reaches a stage requiring invasive therapy.
In this paper, the nature of tissue response to light interaction has been used for early diagnosis, using laser induced fluorescence spectroscopy. Human tooth sample was illuminated with He-Ne laser sources (633 nm) with energy 5 mW. The reflected and emitted spectra of investigated samples were collected using hyperspectral camera to develop multispectral images. The variation of reflected and emitted energy as function in wavelength was employed to generate characteristic spectrum of each tooth tissue. Human teeth caries lesion releases their excess energy by emitting fluorescence light producing chemical footprint signature for each tooth elements and caries state.
This non-invasive, non-contact, and non-ionizing hyperspectral imaging system was employed to diagnose and classify different caries types and stages. Reconstructed 3D fluorescence images offered discrimination between enamel and dentin caries at 633 nm illumination spectral line; white spot lesion was clearly detected and recognized at far visible and infrared wavelength ranges.
This study reports on customized optical imaging system that can offer high sensitivity, high resolution. Optimum stimulating wavelength for early caries detection was reported to be 633 nm. This novel approach can offer a full map of caries degree status.
在病变发展到需要侵入性治疗的阶段之前,必须有一种非侵入性龋齿检测方法来补充临床医生的传统视觉评估。
在本文中,利用组织对光相互作用的反应特性,采用激光诱导荧光光谱法进行早期诊断。用能量为5 mW的氦氖激光源(633 nm)照射人牙样本。使用高光谱相机收集被研究样本的反射光谱和发射光谱,以生成多光谱图像。利用反射和发射能量随波长的变化来生成每个牙齿组织特征光谱。人类牙齿龋损通过发射荧光释放其多余能量,从而为每个牙齿成分和龋损状态产生化学足迹特征。
采用这种非侵入性、非接触且非电离的高光谱成像系统对不同类型和阶段的龋齿进行诊断和分类。重建的三维荧光图像在633 nm照明光谱线下能够区分釉质龋和牙本质龋;在远可见和红外波长范围内能够清晰检测和识别白斑病变。
本研究报告了一种定制的光学成像系统,该系统具有高灵敏度、高分辨率。据报道,早期龋齿检测的最佳激发波长为633 nm。这种新方法能够提供龋齿程度状态的完整图谱。