Leiden University Medical Center, Center for Proteomics and Metabolomics, Albinusdreef 2, 2300RC Leiden, the Netherlands; Leiden University Medical Center, Department of Rheumatology, Albinusdreef 2, 2300RC Leiden, the Netherlands.
Leiden University Medical Center, Department of Rheumatology, Albinusdreef 2, 2300RC Leiden, the Netherlands.
Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Dec;1863(12):1511-1522. doi: 10.1016/j.bbalip.2018.10.003. Epub 2018 Oct 9.
Metabolomics and lipidomics are of fundamental importance to personalized healthcare. Particularly the analysis of bioactive lipids is of relevance to a better understanding of various diseases. Within clinical routines, blood derived samples are widely used for diagnostic and research purposes. Hence, standardized and validated procedures for blood collection and storage are mandatory, in order to guarantee sample integrity and relevant study outcomes. We here investigated different plasma storage conditions and their effect on plasma fatty acid and oxylipid levels. Our data clearly indicate the importance of storage conditions for plasma lipidomic analysis. Storage at very low temperature (-80 °C) and the addition of methanol directly after sampling are the most important measures to avoid ex vivo synthesis of oxylipids. Furthermore, we identified critical analytes being affected under certain storage conditions. Finally, we carried out chiral analysis and found possible residual enzymatic activity to be one of the contributors to the ex vivo formation of oxylipids even at -20 °C.
代谢组学和脂质组学对个性化医疗至关重要。特别是生物活性脂质的分析对于更好地理解各种疾病具有重要意义。在临床常规中，血液样本被广泛用于诊断和研究目的。因此，为了保证样品的完整性和相关研究结果，必须采用标准化和经过验证的采血和储存程序。我们在这里研究了不同的血浆储存条件及其对血浆脂肪酸和氧化脂质水平的影响。我们的数据清楚地表明了储存条件对血浆脂质组学分析的重要性。在非常低的温度（-80°C）下储存，并在采样后直接加入甲醇，是避免氧化脂质体外合成的最重要措施。此外，我们还确定了在某些储存条件下受影响的关键分析物。最后，我们进行了手性分析，发现即使在-20°C下，某些残留的酶活性也可能是氧化脂质体外形成的原因之一。
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