Hirsch P R, Wang C L, Woodward M J
Gene. 1986;48(2-3):203-9. doi: 10.1016/0378-1119(86)90078-8.
A physical and genetic map of the IncP plasmid R1033 was constructed: restriction fragments were subcloned and antibiotic resistance genes were located. The map is consistent with previous reports that R1033 is a derivative of RP4 carrying a 16-kb transposon Tn1696 which contains the antibiotic-resistance determinants present on R1033 but not on RP4. A BamHI fragment from R1033, determining resistance to gentamicin, spectinomycin and streptomycin, was cloned into Tn5, replacing the central Bg/II fragment that determined kanamycin resistance, producing a recombinant transposon Tn5-GmSpSm. This was shown to transpose in Rhizobium leguminosarum at a frequency similar to that of the parental Tn5.
构建了IncP质粒R1033的物理图谱和遗传图谱：对限制酶切片段进行亚克隆，并定位抗生素抗性基因。该图谱与之前的报道一致，即R1033是RP4的衍生物，携带一个16kb的转座子Tn1696，其中包含R1033上存在但RP4上不存在的抗生素抗性决定簇。将R1033中一个决定对庆大霉素、壮观霉素和链霉素抗性的BamHI片段克隆到Tn5中，取代了决定卡那霉素抗性的中央Bg/II片段，产生了一个重组转座子Tn5-GmSpSm。结果表明，该转座子在豆科根瘤菌中的转座频率与亲本Tn5相似。
