Wohlleben W, Arnold W, Bissonnette L, Pelletier A, Tanguay A, Roy P H, Gamboa G C, Barry G F, Aubert E, Davies J
Fakultät für Biologie, Universität Bielefeld, Federal Republic of Germany.
Mol Gen Genet. 1989 Jun;217(2-3):202-8. doi: 10.1007/BF02464882.
The aminoglycoside-3-O-acetyltransferase-I gene (aacC1) from R plasmids of two incompatibility groups (R1033 [Tn1696], and R135) was cloned and sequenced. In the case of R1033, it was shown that the aacC gene is coded by a precise insertion of 833 bp between the aadA promoter and its structural gene in a Tn21 related transposon (Tn1696). This insertion occurs at the same target sequence as that of the OXA-1 beta-lactamase gene insertion in Tn2603. Upstream of the aacC gene, we found an open reading frame (ORF) which is probably implicated in the site-specific recombinational events involved in the evolution of this family of genetic elements. These results provide additional confirmation of the role of Tn21 elements as naturally occurring interspecific transposition and expression cassettes.
对来自两个不相容群（R1033 [Tn1696] 和 R135）的R质粒的氨基糖苷-3-O-乙酰转移酶-I基因（aacC1）进行了克隆和测序。对于R1033，结果表明aacC基因由一个833 bp的精确插入片段编码，该片段插入在与Tn21相关的转座子（Tn1696）中的aadA启动子及其结构基因之间。此插入发生在与Tn2603中OXA-1β-内酰胺酶基因插入相同的靶序列处。在aacC基因上游，我们发现了一个开放阅读框（ORF），它可能与该遗传元件家族进化过程中涉及的位点特异性重组事件有关。这些结果进一步证实了Tn21元件作为天然存在的种间转座和表达盒的作用。
