Department of Orthopaedics Surgery, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, No. 109, Xueyuan West Road, Lucheng District, Wenzhou, 325000, Zhejiang Province, People's Republic of China.
Department of Orthopaedics Surgery, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, No. 75, Jingxiu Road, Lucheng District, Wenzhou, 325000, Zhejiang Province, People's Republic of China.
Biochem Biophys Res Commun. 2018 Nov 2;505(3):945-950. doi: 10.1016/j.bbrc.2018.10.039. Epub 2018 Oct 9.
The study was to investigate the effect of combining treatment with cinnamaldehyde and parathyroid hormone (1-34) (PTH) on glucocorticoid-induced osteoporosis (GIO) and compare with monotherapy. Forty Sprague-Dawley male rats with GIO were divided into four groups randomly: control group (CON group, N = 10); group that intragastric administration with cinnamaldehyde (CIN group, N = 10); group that subcutaneous injection with PTH, three times per week(PTH group, N = 10); both administration with cinnamaldehyde and PTH (CIN + PTH group, N = 10). Distal femurs were harvested for hematoxylin and eosin (H&E) staining, micro-CT scanning and immunohistochemical analysis. Murine mesenchymal stem cells were cultured and dealt with the presence of dexamethasone(DEX group), DEX + cinnamaldehyde(DEX + CIN group), DEX + PTH(DEX + PTH group) or DEX + cinnamaldehyde + PTH(DEX + CIN + PTH group). Alkaline phosphatase (ALP) staining was performed subsequently. The results showed that bone formation in CIN + PTH group was notably promoted compared with other groups. And the expression of tartrate-resistant acid phosphatase (trap) and runt-related transcription factor 2 (runx2) in CIN + PTH group were down-regulated and up-regulated respectively compared with PTH group. In vitro study revealed that ALP-positive cell number in DEX + CIN + PTH group was obviously enhanced compared with other groups. The study revealed that combined treatment with cinnamaldehyde and PTH enhances the therapeutic effect on GIO through inhibiting osteoclastogenesis and promoting osteoblastogenesis.
该研究旨在探讨肉桂醛与甲状旁腺激素（1-34）（PTH）联合治疗对糖皮质激素诱导性骨质疏松症（GIO）的影响，并与单药治疗进行比较。40 只雄性 Sprague-Dawley 大鼠患有 GIO，随机分为四组：对照组（CON 组，N=10）；肉桂醛灌胃组（CIN 组，N=10）；每周皮下注射 PTH 三次（PTH 组，N=10）；肉桂醛和 PTH 联合给药组（CIN+PTH 组，N=10）。取远侧股骨进行苏木精和伊红（H&E）染色、微 CT 扫描和免疫组织化学分析。培养鼠间充质干细胞，并在存在地塞米松（DEX 组）、DEX+肉桂醛（DEX+CIN 组）、DEX+PTH（DEX+PTH 组）或 DEX+肉桂醛+PTH（DEX+CIN+PTH 组）的情况下进行处理。随后进行碱性磷酸酶（ALP）染色。结果表明，与其他组相比，CIN+PTH 组的骨形成明显增强。与 PTH 组相比，CIN+PTH 组的抗酒石酸酸性磷酸酶（trap）和 runt 相关转录因子 2（runx2）的表达分别下调和上调。体外研究表明，与其他组相比，DEX+CIN+PTH 组的 ALP 阳性细胞数明显增加。该研究表明，肉桂醛与 PTH 联合治疗通过抑制破骨细胞生成和促进成骨细胞生成来增强对 GIO 的治疗效果。
