Katoh I, Yoshinaka Y, Sagata N, Ikawa Y
Jpn J Cancer Res. 1987 Feb;78(2):93-8.
We constructed a fusion plasmid, pMX-I, by which the major open reading frame, X-I, of the bovine leukemia virus (BLV) X gene was expressed under control of the mouse metallothionein promoter. pMX-I was cotransfected into CV1 monkey kidney cells together with another construct containing the BLV long terminal repeat (LTR) linked to the chloramphenicol acetyltransferase (CAT) structural gene. The result of assay of CAT synthesis suggests that the X-I product functions as a trans-acting activation factor of the BLV LTR.
我们构建了一个融合质粒pMX-I，通过它，牛白血病病毒（BLV）X基因的主要开放阅读框X-I在小鼠金属硫蛋白启动子的控制下得以表达。pMX-I与另一个构建体一起共转染到CV1猴肾细胞中，该构建体包含与氯霉素乙酰转移酶（CAT）结构基因相连的BLV长末端重复序列（LTR）。CAT合成检测结果表明，X-I产物作为BLV LTR的反式作用激活因子发挥作用。
