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谷氨酸缓解断奶仔猪内毒素攻毒导致的肠道损伤，维持 mTOR 信号通路，抑制 TLR4 和 NOD 信号通路。

Glutamate alleviates intestinal injury, maintains mTOR and suppresses TLR4 and NOD signaling pathways in weanling pigs challenged with lipopolysaccharide.

机构信息

Hubei Key Laboratory of Animal Nutrition and Feed Science, Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Wuhan Polytechnic University, Wuhan, 430023, P. R. China.

Zhe Jiang Goshine Test Technologies Co., Ltd., Hangzhou, 310030, P. R. China.

出版信息

Sci Rep. 2018 Oct 11;8(1):15124. doi: 10.1038/s41598-018-33345-7.

DOI:10.1038/s41598-018-33345-7
PMID:30310102
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6181909/
Abstract

This experiment aimed to explore whether glutamate (Glu) had beneficial effects on intestinal injury caused by Escherichia coli LPS challenge via regulating mTOR, TLRs, as well as NODs signaling pathways. Twenty-four piglets were allotted to 4 treatments including: (1) control group; (2) LPS group; (3) LPS + 1.0% Glu group; (4) LPS + 2.0% Glu group. Supplementation with Glu increased jejunal villus height/crypt depth ratio, ileal activities of lactase, maltase and sucrase, and RNA/DNA ratio and protein abundance of claudin-1 in jejunum and ileum. In addition, the piglets fed Glu diets had higher phosphorylated mTOR (Ser)/total mTOR ratio in jejunum and ileum. Moreover, Glu decreased TNF-α concentration in plasma. Supplementation with Glu also decreased mRNA abundance of jejunal TLR4, MyD88, IRAK1, TRAF6, NOD2 and increased mRNA abundance of ileal Tollip. These results indicate that Glu supplementation may be closely related to maintaining mTOR and inhibiting TLR4 and NOD signaling pathways, and concomitant improvement of intestinal integrity under an inflammatory condition.

摘要

本实验旨在探讨谷氨酸（Glu）是否通过调节 mTOR、TLRs 和 NODs 信号通路对大肠杆菌 LPS 刺激引起的肠道损伤有有益作用。将 24 头仔猪分配到 4 种处理中，包括：（1）对照组；（2）LPS 组；（3）LPS+1.0%Glu 组；（4）LPS+2.0%Glu 组。添加 Glu 可增加空肠绒毛高度/隐窝深度比、回肠乳糖酶、麦芽糖酶和蔗糖酶活性以及空肠和回肠的 Claudin-1 的 RNA/DNA 比和蛋白丰度。此外，饲喂 Glu 日粮的仔猪空肠和回肠中磷酸化 mTOR（Ser）/总 mTOR 比值更高。此外，Glu 降低了血浆中 TNF-α 的浓度。添加 Glu 还降低了空肠 TLR4、MyD88、IRAK1、TRAF6、NOD2 的 mRNA 丰度，增加了回肠 Tollip 的 mRNA 丰度。这些结果表明，Glu 的添加可能与维持 mTOR 以及抑制 TLR4 和 NOD 信号通路密切相关，并在炎症条件下伴随改善肠道完整性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/6181909/0b03e322e6a8/41598_2018_33345_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/6181909/72c335b1cafb/41598_2018_33345_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/6181909/8d1ec3df0bfa/41598_2018_33345_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/6181909/0b03e322e6a8/41598_2018_33345_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/6181909/72c335b1cafb/41598_2018_33345_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/6181909/8d1ec3df0bfa/41598_2018_33345_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/276d/6181909/0b03e322e6a8/41598_2018_33345_Fig3_HTML.jpg

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