Rosenberg J, Pines M, Hurwitz S
J Endocrinol. 1987 Mar;112(3):431-7. doi: 10.1677/joe.0.1120431.
Dispersed adrenal cells of avian and bovine origin were incubated with human (h) 1-34 parathyroid hormone (PTH(1-34)), bovine (b) PTH(1-84), bPTH(3-34), avian (a) PTH, and with ACTH. Kidney tubular cells, the established target cells for PTH, were used for comparison. Cyclic AMP (cAMP) accumulation and steroid hormone (aldosterone and corticosterone) secretion were measured in response to the hormones. In the bovine adrenocortical cells PTH of both bovine and avian origin stimulated cAMP production, but the aPTH action was more pronounced. The maximal cAMP response of avian adrenocortical cells to aPTH was 15-fold greater than that of ACTH, but a 20-times higher concentration of aPTH was required to reach half-maximal response. Avian PTH stimulated steroid hormone secretion in the chick adrenocortical cells, but the induced secretion was similar to that induced by ACTH, despite the difference in cAMP accumulation. Human PTH(1-34) and bPTH(1-84), which stimulated cAMP production in kidney cells, and the conventional antagonist bPTH(3-34) inhibited aPTH stimulation of cAMP accumulation in avian adrenocortical cells, but did not interfere with ACTH action. Furthermore, cAMP stimulation by aPTH and ACTH in avian adrenal cells was additive. The results establish the adrenal as a target organ for PTH, and suggest that the PTH acts through specific receptors, distinct from those for ACTH.
将源自禽类和牛类的分散肾上腺细胞与人（h）1-34甲状旁腺激素（PTH(1-34)）、牛（b）PTH(1-84)、bPTH(3-34)、禽类（a）PTH以及促肾上腺皮质激素（ACTH）一起孵育。肾小管细胞是已确定的PTH靶细胞，用作对照。检测了细胞对这些激素的反应中，环磷酸腺苷（cAMP）的积累以及类固醇激素（醛固酮和皮质酮）的分泌情况。在牛肾上腺皮质细胞中，源自牛类和禽类的PTH均刺激了cAMP的产生，但aPTH的作用更为明显。禽类肾上腺皮质细胞对aPTH的最大cAMP反应比ACTH的大15倍，但达到半最大反应所需的aPTH浓度要高20倍。禽类PTH刺激了鸡肾上腺皮质细胞中类固醇激素的分泌，尽管cAMP积累有所不同，但诱导的分泌与ACTH诱导的相似。人PTH(1-34)和bPTH(1-84)可刺激肾细胞中cAMP的产生，而传统拮抗剂bPTH(3-34)可抑制aPTH对禽类肾上腺皮质细胞中cAMP积累的刺激作用，但不干扰ACTH的作用。此外，aPTH和ACTH对禽类肾上腺细胞中cAMP的刺激作用具有加和性。这些结果确立了肾上腺是PTH的靶器官，并表明PTH通过与ACTH不同的特异性受体发挥作用。
