Sireesha Tanniru, Asha Syed, Malathi Jojula
Dept. of Bio-Technology, VFSTR (Deemed to be University), Vadlamudi, Guntur-522017, India.
Department of Microbiology, Sri Shivani College of Pharmacy, Warangal, India.
Infect Disord Drug Targets. 2019;19(2):185-192. doi: 10.2174/1871526518666181011152914.
Tuberculosis (TB) among Diabetes Mellitus (DM) population is more expected to fail treatment of TB due to smear-negative TB.
We sought to compare and evaluate different methods to study the frequency of TB infections among DM patients.
Blood, sputum and urine samples were collected from 500 newly identified diabetic patients from different diabetic clinics in Warangal districts. Smear microscopy, Culture and Line Probe Assay by Polymerase Chain Reaction (LPA PCR) were used for identification of tuberculosis.
Based on the chest X-Ray of 200 diabetic patients, suspected with pulmonary infections, 113 were males, 85 were females and 2 were children. All 200 patients were tested for tuberculosis infections, 55 were confirmed based on chest X-ray lesions. Off 55 patients, 30 were positive and 25 were negative for AFB microscopy, but were shown positive for chest X-ray. 22 were reported to be culture positive on solid media and identified as Mycobacterium tuberculosis based on morphology and biochemical methods. 36 samples were identified to be positive for LED, FM microscopy and LPA. Off 36 positive samples, 2 were MDR-TB and 34 were MTB based on LPA PCR method. Off 25 smear negative samples, 2 were identified as culture positive and confirmed to be MTB by morphological, biochemical tests.
Smear Negative Microscopy plays a vital role in the spread of tuberculosis infection among diabetic patients. Along with the smear microscopy, there is a need to rely on other methods for rapid identification and diagnosis of tuberculosis among the diabetic patients to control the spread of infection in the community and household contacts.
糖尿病（DM）人群中的结核病（TB）因涂片阴性肺结核更易治疗失败。
我们试图比较和评估不同方法以研究糖尿病患者中结核感染的频率。
从瓦朗加尔地区不同糖尿病诊所的500名新确诊糖尿病患者中采集血液、痰液和尿液样本。采用涂片显微镜检查、培养及聚合酶链反应线性探针测定法（LPA PCR）鉴定结核病。
基于200例疑似肺部感染的糖尿病患者的胸部X线检查，其中113例为男性，85例为女性，2例为儿童。对所有200例患者进行结核感染检测，55例根据胸部X线病变确诊。在这55例患者中，30例抗酸杆菌（AFB）显微镜检查呈阳性，25例呈阴性，但胸部X线检查呈阳性。据报告，22例在固体培养基上培养呈阳性，根据形态学和生化方法鉴定为结核分枝杆菌。36份样本LED、荧光显微镜检查和LPA呈阳性。在36份阳性样本中，根据LPA PCR方法，2例为耐多药结核病（MDR-TB），34例为结核分枝杆菌（MTB）。在25份涂片阴性样本中，2例培养呈阳性，经形态学、生化检测确认为MTB。
涂片阴性显微镜检查在糖尿病患者结核感染传播中起重要作用。除涂片显微镜检查外，还需要依靠其他方法快速鉴定和诊断糖尿病患者中的结核病，以控制社区和家庭接触者中的感染传播。
