肠杆菌科碳青霉烯酶检测试剂盒（EntericBio CPE assay）用于检测产碳青霉烯酶的生物体的评估。
Evaluation of the EntericBio CPE assay for the detection of carbapenemase-producing organisms.
机构信息
1HSL Analytics LLP, 1 Mabledon Place, London, WC1H 9AJ, UK.
2The Royal Free London NHS Foundation Trust, Pond Street, London, NW3 2QG, UK.
出版信息
J Med Microbiol. 2018 Dec;67(12):1728-1730. doi: 10.1099/jmm.0.000851. Epub 2018 Oct 15.
Carbapenemase-producing organisms (CPOs) represent an increasing threat in healthcare facilities and detection of these organisms in the diagnostic laboratory can be challenging. The EntericBio CPE (EBCPE) real-time PCR assay (Serosep Ltd) was evaluated for the detection of NDM, KPC, OXA-48-like, VIM, IMP and GES carbapenemase genes from a panel of 145 multidrug-resistant organisms (29 NDM, 35 OXA-48, 21 VIM, 4 OXA-23, 3 KPC, 5 NDM+OXA-48, 3 GES-5, 1 OXA-23+NDM, 1 IMI, 2 IMP-1 and 41 multidrug-resistant carbapenemase-negative isolates). The EBCPE assay performed well, with 100 % sensitivity and specificity for the detection of all genotypes included in the assay. Turnaround time and laboratory workflow were improved compared to culture-based assays. Users must remain aware of the limitations of molecular assays for CPO detection to ensure implementation of the most suitable CPO diagnostic pathways.
产碳青霉烯酶的生物体（CPO）在医疗机构中构成的威胁日益增加，在诊断实验室中检测这些生物体具有一定挑战性。本文评估了 Serosep 有限公司的 EntericBio CPE（EBCPE）实时 PCR 检测法，用于检测来自 145 株多药耐药菌（29 株 NDM、35 株 OXA-48、21 株 VIM、4 株 OXA-23、3 株 KPC、5 株 NDM+OXA-48、3 株 GES-5、1 株 OXA-23+NDM、1 株 IMI、2 株 IMP-1 和 41 株多药耐药但碳青霉烯酶阴性的分离株）的 NDM、KPC、OXA-48 样、VIM、IMP 和 GES 碳青霉烯酶基因。EBCPE 检测法性能良好，对于检测该检测法中包含的所有基因型，其灵敏度和特异性均为 100%。与基于培养的检测法相比，其周转时间和实验室工作流程得到了改善。用户必须意识到分子检测法在检测 CPO 方面的局限性，以确保实施最合适的 CPO 诊断途径。
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