California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA, 94720, USA.
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 94720, USA.
Chembiochem. 2019 Jan 2;20(1):7-19. doi: 10.1002/cbic.201800478. Epub 2018 Nov 8.
Heme-nitric oxide/oxygen binding (H-NOX) proteins are a family of gas-binding hemoproteins that bind diatomic gas ligands such as nitric oxide (NO) and oxygen (O ). In bacteria, H-NOXs are often associated with signaling partners, including histidine kinases (HKs), diguanylate cyclases (DGCs) or methyl-accepting chemotaxis proteins (MCPs), either as a stand-alone protein or as a domain of a larger polypeptide. H-NOXs regulate the activity of cognate signaling proteins through ligand-induced conformational changes in the H-NOX domain and protein/protein interactions between the H-NOX and the cognate signaling partner. This review summarizes recent progress toward deciphering the molecular mechanism of bacterial H-NOX activation and the subsequent regulation of H-NOX-associated cognate sensor proteins from a structural and biochemical point of view.
血红素-一氧化氮/氧结合（H-NOX）蛋白是一类结合双原子气体配体（如一氧化氮（NO）和氧（O ））的气体结合血红素蛋白。在细菌中，H-NOX 通常与信号伴侣（包括组氨酸激酶（HK）、双鸟苷酸环化酶（DGC）或甲基接受趋化蛋白（MCP））相关联，作为独立的蛋白质或作为较大多肽的一个结构域。H-NOX 通过在 H-NOX 结构域中诱导配体诱导的构象变化以及 H-NOX 和同源信号伴侣之间的蛋白质/蛋白质相互作用来调节同源信号蛋白的活性。本综述从结构和生化角度总结了近年来在破译细菌 H-NOX 激活的分子机制以及随后对 H-NOX 相关同源传感器蛋白的调节方面的最新进展。
