Instituto de Ecorregiones Andinas (INECOA), Universidad Nacional de Jujuy, CONICET, San Salvador de Jujuy, Argentina.
J Exp Zool A Ecol Integr Physiol. 2019 Feb;331(2):93-102. doi: 10.1002/jez.2240. Epub 2018 Oct 15.
Light pollution or artificial lighting at night (ALAN) is an emerging threat to biodiversity that can disrupt physiological processes and behaviors. Because ALAN stressful effects are little studied in diurnal amphibian species, we investigated if chronic ALAN exposure affects the leukocyte profile, body condition, and blood cell sizes of a diurnal toad. We hand-captured male toads of Melanophryniscus rubriventris in Angosto de Jaire (Jujuy, Argentina). We prepared blood smears from three groups of toads: "field" (toads processed in the field immediately after capture), "natural light" (toads kept in the laboratory under captivity with natural photoperiod), and "constant light" (toads kept in the laboratory under captivity with constant photoperiod/ALAN). We significantly observed higher neutrophil proportions and neutrophils to lymphocytes ratio in toads under constant light treatment. In addition, we observed significantly better body condition and higher erythrocyte size in field toads compared with captive toads. In summary, ALAN can trigger a leukocyte response to stress in males of the diurnal toad M. rubriventris. In addition, captivity can affect the body condition and erythrocyte size of these toads.
光污染或夜间人工照明（ALAN）是对生物多样性的一种新出现的威胁，它可以扰乱生理过程和行为。由于 ALAN 的应激效应在昼行性两栖动物物种中研究甚少，我们研究了慢性 ALAN 暴露是否会影响昼行性蟾蜍的白细胞谱、身体状况和血细胞大小。我们在安戈斯托·德·杰尔（胡胡伊省，阿根廷）手捕到了雄性 M. rubriventris 蟾蜍。我们从三组蟾蜍中制备了血涂片：“野外”（捕获后立即在野外处理的蟾蜍）、“自然光”（在实验室中被囚禁，有自然光周期）和“持续光照”（在实验室中被囚禁，有持续光照/ALAN）。我们发现，在持续光照处理下，蟾蜍的中性粒细胞比例和中性粒细胞与淋巴细胞比值显著升高。此外，与被囚禁的蟾蜍相比，野外的蟾蜍身体状况更好，红细胞尺寸更大。总之，ALAN 会引发昼行性蟾蜍 M. rubriventris 雄性的白细胞应激反应。此外，囚禁会影响这些蟾蜍的身体状况和红细胞大小。
