Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald. Felix-Hausdorff-Str. 8, Greifswald 17475, Germany; Department of Medical Microbiology, University of Groningen, Hanzeplein 1, P.O. Box 30001, Groningen 9700, the Netherlands.
Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald. Felix-Hausdorff-Str. 8, Greifswald 17475, Germany.
J Proteomics. 2019 Feb 20;193:154-161. doi: 10.1016/j.jprot.2018.10.005. Epub 2018 Oct 12.
Methicillin-resistant Staphylococcus aureus (MRSA) originally emerged in nosocomial settings and has subsequently spread into the community. In turn, community-associated (CA) MRSA lineages are nowadays introduced from the community into hospitals where they can cause hospital-associated (HA) infections. This raises the question of how the CA-MRSA lineages adapt to the hospital environment. Previous studies implicated particular virulence factors in the CA-behaviour of MRSA. However, we hypothesized that physiological changes may also impact staphylococcal epidemiology. With the aim to identify potential metabolic adaptations, we comparatively profiled the cytosolic proteomes of CA- and HA-isolates from the USA300 lineage that was originally identified as CA-MRSA. Interestingly, enzymes for gluconeogenesis, the tricarboxylic acid cycle and biosynthesis of amino acids are up-regulated in the investigated CA-MRSA isolates, while enzymes for glycolysis and the pentose phosphate pathway are up-regulated in the HA-MRSA isolates. Of note, these data apparently match with the clinical presentation of each group. These observations spark interest in central carbon metabolism as a key driver for adaptations that streamline MRSA for propagation in the community or the hospital.
耐甲氧西林金黄色葡萄球菌（MRSA）最初出现在医院环境中，随后传播到社区。相反，现在社区相关（CA）MRSA 谱系从社区引入医院，在那里它们可能导致医院相关（HA）感染。这就提出了一个问题，即 CA-MRSA 谱系如何适应医院环境。先前的研究表明，某些毒力因子与 MRSA 的 CA 行为有关。然而，我们假设生理变化也可能影响葡萄球菌的流行病学。为了确定潜在的代谢适应，我们比较了源自美国 300 谱系的 CA 和 HA 分离株的细胞质蛋白质组，该谱系最初被确定为 CA-MRSA。有趣的是，参与糖异生、三羧酸循环和氨基酸生物合成的酶在研究的 CA-MRSA 分离株中上调，而参与糖酵解和戊糖磷酸途径的酶在 HA-MRSA 分离株中上调。值得注意的是，这些数据显然与每组的临床表现相匹配。这些观察结果引发了人们对中心碳代谢的兴趣，认为它是推动 MRSA 在社区或医院中传播的关键驱动力。
