多黏菌素B九肽（PMBN）对转化的影响。

The effect of polymyxin B nonapeptide (PMBN) on transformation.

作者信息

Viljanen P

出版信息

Biochem Biophys Res Commun. 1987 Mar 30;143(3):923-7. doi: 10.1016/0006-291x(87)90338-x.

Abstract

The effect of the outer membrane permeabilizing polycation, polymyxin B nonapeptide (PMBN) on the transformation of E. coli HB101 with pBR322 plasmid DNA was investigated. Pretreatment of cells with PMBN (followed by suspending the cells in PMBN-free medium) did not stimulate the development of competence induced by the calcium heat pulse. In the absence of calcium-ions, a high PMBN concentration (1 mM) was able to induce a low transformation frequency provided that PMBN was not removed before the addition of DNA.

摘要

研究了外膜通透化聚阳离子多粘菌素B九肽（PMBN）对大肠杆菌HB101用pBR322质粒DNA进行转化的影响。用PMBN预处理细胞（随后将细胞悬浮于不含PMBN的培养基中）并不能刺激由钙热脉冲诱导的感受态的形成。在没有钙离子的情况下，高浓度的PMBN（1 mM）能够诱导低转化频率，前提是在加入DNA之前不去除PMBN。