Sinha B K
Biochim Biophys Acta. 1987 May 19;924(2):261-9. doi: 10.1016/0304-4165(87)90021-3.
Spin-trapping techniques and electron spin resonance spectroscopy have been used to study bioactivations of hydrazine and its derivatives by isolated perfused rat livers. Using phenylbutylnitrone (PBN) as the stable spin trap, it was found that the liver perfusion of hydrazine, acetylhydrazine and isoniazid resulted in the formation of the same carbon-centered radical which was shown to be the acetyl radical. The identity of the acetyl radical was confirmed after organic extraction of the liver perfusates, by comparing its coupling constants with those of the in vitro metal ion- or horseradish peroxidase-catalyzed oxidation products of the hydrazines in the same solvents. The liver perfusion of iproniazid formed the isopropyl radical which was previously observed to result from peroxidase-catalyzed oxidation.
自旋捕集技术和电子自旋共振光谱已被用于研究肼及其衍生物在离体灌注大鼠肝脏中的生物活化作用。使用苯基丁基硝酮（PBN）作为稳定的自旋捕集剂，发现用肼、乙酰肼和异烟肼灌注肝脏会导致形成相同的以碳为中心的自由基，该自由基被证明是乙酰基自由基。通过将肝脏灌注液经有机萃取后其耦合常数与相同溶剂中肼的体外金属离子或辣根过氧化物酶催化氧化产物的耦合常数进行比较，证实了乙酰基自由基的身份。用异丙烟肼灌注肝脏形成了异丙基自由基，该自由基先前已观察到是由过氧化物酶催化氧化产生的。
