Mallefet Jérôme, Duchatelet Laurent, Hermans Claire, Baguet Fernand
Catholic University of Louvain-La-Neuve, Marine Biology Laboratory, 3 Place croix du Sud, 1348, Louvain-La-Neuve, Belgium.
Catholic University of Louvain-La-Neuve, Marine Biology Laboratory, 3 Place croix du Sud, 1348, Louvain-La-Neuve, Belgium.
Acta Histochem. 2019 Jan;121(1):7-15. doi: 10.1016/j.acthis.2018.10.001. Epub 2018 Oct 12.
Nervous control of light emission from deep-sea mesopelagic fishes has been documented for several species. Studies on the nervous control of photophores from deep-sea luminescent fish, are mainly restricted to a pharmacological approach. For example, the light organs, called photophores, isolated from Argyropelecus hemygimnus and Maurolicus muelleri show a much higher sensitivity to adrenaline than to noradrenaline. According to these results and other information in different species, catecholamines are considered as main neurotransmitters triggering bioluminescence in deep-sea fishes. The present work is a study of the nervous control of the isolated photophores from two Stomiid fishes, Chauliodus sloani (the viperfish) and Stomias boa (the dragonfish) with the aim to determine the nature of the nervous control by pharmacological, biochemical and morphological approaches. Results show that, although the photophores of both species are sensitive to catecholamines, adrenaline is present in larger amount than noradrenaline in the light organs of C. sloani. Both catecholamines have different immunoreactive (IR) sites, noradrenaline showing a very diffuse localization as compared to adrenaline in C. sloani. On the contrary, only adrenaline is detected in the photocytes chamber and nerves innervating the photophore in S. boa. Knowing that the majority of dragonfishes exhibit a luminescent chin barbel, we also investigated the presence of catecholamines in this specific tissue in S. boa. Immunohistology reveals the presence of adrenaline within the tissue forming the chin barbel; adrenaline-IR is found in the connective tissue surroundings two group of muscle fibers and blood vessels in the stem but also around the multiple blood vessels located within the barbel bulb. Our results strongly support the adrenergic control of light emission in bioluminescent stomiid fishes.
已有文献记载了几种深海中层鱼类发光的神经控制情况。对深海发光鱼类发光器神经控制的研究，主要局限于药理学方法。例如，从半光银斧鱼和穆氏钻光鱼分离出的发光器官（称为发光器），对肾上腺素的敏感性比对去甲肾上腺素高得多。根据这些结果以及不同物种的其他信息，儿茶酚胺被认为是触发深海鱼类生物发光的主要神经递质。本研究旨在通过药理学、生物化学和形态学方法，研究两种巨口鱼（斯氏蝰鱼和博氏巨口鱼）分离出的发光器的神经控制，以确定神经控制的性质。结果表明，虽然这两个物种的发光器对儿茶酚胺都敏感，但在斯氏蝰鱼的发光器官中，肾上腺素的含量比去甲肾上腺素多。两种儿茶酚胺具有不同的免疫反应（IR）位点，与斯氏蝰鱼中的肾上腺素相比，去甲肾上腺素显示出非常分散的定位。相反，在博氏巨口鱼的发光细胞腔和支配发光器的神经中只检测到肾上腺素。鉴于大多数巨口鱼都有发光的颏须，我们还研究了博氏巨口鱼这种特定组织中儿茶酚胺的存在情况。免疫组织学显示，在构成颏须的组织中存在肾上腺素；在颏须茎部围绕两组肌肉纤维和血管的结缔组织中，以及在颏须球体内的多条血管周围，都发现了肾上腺素免疫反应。我们的结果有力地支持了发光巨口鱼发光的肾上腺素能控制。
