Rehman Rehana, Ashraf Mussarat, Jasmine Anila, Lal Karishma, Alam Faiza
Department of Biological and Biomedical Sciences, Aga Khan Medical College, Karachi, Pakistan.
Aga Khan Medical College, Karachi, Pakistan.
J Pak Med Assoc. 2018 Jun;68(6):862-866.
To evaluate the role of tumour necrosis factor alpha and interleukin-6 in conception after assisted reproductive treatment.
The longitudinal cohort study was conducted from August 2014 to May 2015 and comprised patients undergoing intracytoplasmic sperm injection at the Islamabad Clinic Serving Infertile Couples, Islamabad, Pakistan. During the procedure, endometrial thickness was measured on the day of ovulation induction and serum tumour necrosis factor alpha along with interleukin-6 were measured on the embryo transfer day. Subjects were divided into groups, with non-pregnant females in Group A and those with confirmed foetal cardiac activity in Group B and non-pregnant females were divided into two groups. The difference between the groups and the association of cytokines with endometrial thickness were measured. .
Of the 131 subjects, 79(60%) were in Group A and 52(40%) in Group-B. The mean level of interleukin-6 was 49.65±3.04 in Group B and 104.14±76.03 in Group A, with significant inverse correlation with endometrial thickness (p<0.001). The level of tumour necrosis factor alpha were significantly higher in Group-B compared to Group-A (p<0.001).
The pro-inflammatory cytokine, tumour necrosis factor alpha, takes part in preparation of endometrial lining for implantation of embryo in assisted reproductive treatment procedures.
评估肿瘤坏死因子α和白细胞介素-6在辅助生殖治疗后受孕中的作用。
2014年8月至2015年5月进行了纵向队列研究，纳入了在巴基斯坦伊斯兰堡不育夫妇诊所接受卵胞浆内单精子注射的患者。在该过程中，在促排卵当天测量子宫内膜厚度，并在胚胎移植当天测量血清肿瘤坏死因子α和白细胞介素-6。将受试者分组，A组为未怀孕女性，B组为确认有胎儿心脏活动的女性，未怀孕女性再分为两组。测量组间差异以及细胞因子与子宫内膜厚度的相关性。
131名受试者中，A组79人（60%），B组52人（40%）。B组白细胞介素-6的平均水平为49.65±3.04，A组为104.14±76.03，与子宫内膜厚度呈显著负相关（p<0.001）。B组肿瘤坏死因子α水平显著高于A组（p<0.001）。
促炎细胞因子肿瘤坏死因子α参与辅助生殖治疗过程中子宫内膜为胚胎着床做准备的过程。
