Stichel C C, Singer W
J Comp Neurol. 1987 Apr 1;258(1):91-8. doi: 10.1002/cne.902580107.
The laminar distribution of cholinergic axons was analyzed quantitatively in the visual cortex of adult cats by using immunocytochemistry with a monoclonal antibody against choline acetyltransferase (ChAT). ChAT(+) fibers and varicosities were counted at different locations within area 17 and the distribution patterns in various animals were compared. Choline acetyltransferase-immunoreactivity was localized in fine, varicose fibers, which were present in all layers of the visual cortex. The density of labeled fibers was highest in layer I, which contained 14% of all fibers and 19.5% of all varicosities, and decreased toward deeper layers. The number of varicosities decreased more markedly toward deeper layers than the frequency of fibers. These distribution patterns were very consistent, showing only slight intra- and interindividual variability.
通过使用针对胆碱乙酰转移酶（ChAT）的单克隆抗体进行免疫细胞化学分析，对成年猫视觉皮层中胆碱能轴突的分层分布进行了定量分析。在17区的不同位置对ChAT（+）纤维和曲张体进行计数，并比较了不同动物的分布模式。胆碱乙酰转移酶免疫反应定位于视觉皮层各层中存在的细小、曲张纤维。标记纤维的密度在I层最高，该层包含所有纤维的14%和所有曲张体的19.5%，并向深层逐渐降低。曲张体的数量比纤维的频率向深层减少得更明显。这些分布模式非常一致，个体内和个体间仅表现出轻微的变异性。
