抗肿瘤药物的光敏作用2：蒽吡唑对维生素C和3,4-二羟基苯丙氨酸的光敏氧化作用

Photosensitization by antitumor agents 2: anthrapyrazole-photosensitized oxidation of ascorbic acid and 3,4-dihydroxyphenylalanine.

作者信息

Reszka K, Kolodziejczyk P, Lown J W

出版信息

J Free Radic Biol Med. 1986;2(3):203-11. doi: 10.1016/s0748-5514(86)80071-x.

Abstract

A novel anthrapyrazole anticancer agent has been examined for photosensitizing properties. Illumination of the anthrapyrazole and ascorbic acid with blue light in aerated aqueous solutions causes SOD and catalase-sensitive oxygen consumption, indicating involvement of both superoxide radical and hydrogen peroxide in this process. Electron paramagnetic resonance showed that the ascorbyl radical is also produced during the photooxidation. When 3,4-dihydroxyphenylalanine (Dopa) is used as a substrate, production of hydrogen peroxide is evidenced by catalase-sensitive oxygen consumption. Generation of hydroxyl radicals during illumination of the drug and ascorbic acid (or Dopa) in the presence of catalytic amounts of the Fe(III)/EDTA complex is demonstrated using EPR and spin-trapping techniques.

摘要

一种新型蒽并吡唑类抗癌剂已被研究其光敏特性。在充气水溶液中用蓝光照射蒽并吡唑和抗坏血酸会导致超氧化物歧化酶（SOD）和过氧化氢酶敏感的氧气消耗，表明超氧自由基和过氧化氢都参与了这一过程。电子顺磁共振显示在光氧化过程中也会产生抗坏血酸自由基。当使用3,4-二羟基苯丙氨酸（多巴）作为底物时，过氧化氢酶敏感的氧气消耗证明了过氧化氢的产生。使用电子顺磁共振（EPR）和自旋捕获技术证明，在催化量的Fe(III)/EDTA络合物存在下，光照药物和抗坏血酸（或多巴）时会产生羟基自由基。