Berkow R L, Dodson R W, Kraft A S
J Leukoc Biol. 1987 May;41(5):441-6. doi: 10.1002/jlb.41.5.441.
The role of C-kinase in the activation of human polymorphonuclear leukocytes has been examined using H-7, a recently described C-kinase inhibitor. We found that H-7 will inhibit PMN superoxide anion release in response to the tumor promotor phorbol myristate acetate and the calcium ionophore A23187. In contrast, no inhibition by H-7 was seen for PMN superoxide release stimulated by the chemotactic peptide FMLP. H-7 did not inhibit PMN NADPH oxidase activity or PMN degranulation by any stimulant, but it reversed a phorbol ester-induced inhibition of granule release by FMLP. The results show that H-7 differentially affects the PMN functional events of secretion and superoxide release and suggests that an H-7 inhibitable C-kinase is not involved in chemotactic peptide induced activation of PMN and may not regulate stimulus induced PMN degranulation.
利用最近描述的一种C激酶抑制剂H - 7，研究了C激酶在人多形核白细胞激活中的作用。我们发现H - 7会抑制多形核白细胞对肿瘤促进剂佛波酯肉豆蔻酸酯乙酸盐和钙离子载体A23187的超氧阴离子释放。相比之下，对于趋化肽FMLP刺激的多形核白细胞超氧释放，未观察到H - 7的抑制作用。H - 7不抑制任何刺激剂引起的多形核白细胞NADPH氧化酶活性或多形核白细胞脱颗粒，但它逆转了佛波酯诱导的FMLP对颗粒释放的抑制作用。结果表明，H - 7对多形核白细胞分泌和超氧释放的功能事件有不同影响，并表明一种可被H - 7抑制的C激酶不参与趋化肽诱导的多形核白细胞激活，可能也不调节刺激诱导的多形核白细胞脱颗粒。
