Li Chong, Zhang Panpan, Hao Yanming, He Dawei, Shen Yixin, Lu Rongzhu
Department of Orthopedics, The First People's Hospital of Kunshan, Jiangsu University, Kunshan, Jiangsu 215300, P.R. China.
Department of Laboratory, The First People's Hospital of Kunshan, Jiangsu University, Kunshan, Jiangsu 215300, P.R. China.
Oncol Lett. 2018 Nov;16(5):5997-6002. doi: 10.3892/ol.2018.9362. Epub 2018 Aug 24.
The aim of the present study was to investigate the clinical application and utility of CdSe/ZnS quantum dots (QDs) in tracing RAW 264.7 macrophages. RAW 264.7 cells and QDs at various concentrations were co-cultured for 24 h, and the fluorescence intensity of the macrophages was determined at various time points. The mRNA expression levels of genes encoding inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1β] were determined, and cellular assays were performed to investigate the activation, proliferation and apoptosis of RAW 264.7 cells. The QDs were engulfed by the macrophages, and the fluorescence intensity of RAW 264.7 cells increased with increasing concentration and time. The IL-1β mRNA level increased significantly at 50 µg/ml QDs, and that of TNF-α increased significantly at 100 µg/ml QDs. Accelerated proliferation of RAW 264.7 cells was observed at 50 and 100 µg/ml QDs; however, no increase in apoptosis of RAW 264.7 cells was observed in co-culture. CdSe/ZnS QDs may be used as tracers due to the fluorescence intensity of RAW 264.7 cells increasing with increasing QD concentration and time, resulting in the activation of macrophages and significant increases in proliferation at 50 and 100 µg/ml QDs compared with in the absence of QDs. The change in QD concentration was not significantly associated with the proliferation and apoptosis of RAW 264.7 macrophages.
本研究的目的是探讨CdSe/ZnS量子点（QDs）在追踪RAW 264.7巨噬细胞中的临床应用及效用。将RAW 264.7细胞与不同浓度的量子点共培养24小时，并在不同时间点测定巨噬细胞的荧光强度。测定编码炎性细胞因子[肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β]的基因的mRNA表达水平，并进行细胞实验以研究RAW 264.7细胞的激活、增殖和凋亡。量子点被巨噬细胞吞噬，RAW 264.7细胞的荧光强度随浓度和时间的增加而增加。在量子点浓度为50μg/ml时，IL-1β的mRNA水平显著升高，在量子点浓度为100μg/ml时，TNF-α的mRNA水平显著升高。在量子点浓度为50和100μg/ml时观察到RAW 264.7细胞增殖加速；然而，在共培养中未观察到RAW 264.7细胞凋亡增加。由于RAW 264.7细胞的荧光强度随量子点浓度和时间的增加而增加，与无量子点时相比，在量子点浓度为50和100μg/ml时巨噬细胞被激活且增殖显著增加，因此CdSe/ZnS量子点可作为示踪剂。量子点浓度的变化与RAW 264.7巨噬细胞的增殖和凋亡无显著相关性。
