Respiratory Diseases, University of Bari, Italy.
Pulmonology, Ospedale Di Venere, Bari, Italy.
J Biol Regul Homeost Agents. 2018 Sep-Oct;32(5):1261-1265.
Electronic noses (e-noses) are a cheap and easy method for exhaled Volatile Organic Compound (VOC)-analysis which has shown its potential in several diseases. Before obtaining a full validation of these instruments in clinical settings, a number of methodological issues still have to be established. We aimed to investigate a potential influence of circadian variation on VOC-profile analyzed by an e-nose in healthy subjects. We enrolled 22 adults free of any known diseases. A sequence of exhaled breath samplings were performed on all participants at predetermined hours (7am, 12pm, 17pm, 23pm) and analyzed by an e-nose (Cyranose 320). According to Principal Component Analysis, significant circadian variations of the exhaled VOC-profile were shown for Principal Component (PC) 1 and 3. In detail, PC1 and PC3 values were significantly higher in the morning compared to the afternoon and evening (for all parameters p less than 0.05). Successive Linear Discriminant analysis confirmed the findings above. The daily variations in VOCs-profile, with the peak in the morning, could be relevant for future clinical applications, especially in the choice of optimal time for sampling patients.
电子鼻（e-nose）是一种廉价且易于使用的呼气挥发性有机化合物（VOC）分析方法，已在多种疾病中显示出其潜力。在这些仪器在临床环境中得到充分验证之前，仍需要确定一些方法学问题。我们旨在研究健康受试者中电子鼻分析的 VOC 谱是否存在昼夜节律变化的影响。我们招募了 22 名无任何已知疾病的成年人。所有参与者都在预定时间（7 点、12 点、17 点、23 点）进行一系列呼气采样，并由电子鼻（Cyranose 320）进行分析。根据主成分分析，呼气 VOC 谱的主要成分（PC）1 和 3 显示出明显的昼夜节律变化。具体来说，与下午和晚上相比，PC1 和 PC3 的值在早上明显更高（所有参数 p 值均小于 0.05）。连续线性判别分析证实了上述发现。VOCs 谱的日间变化，早晨出现峰值，可能与未来的临床应用相关，尤其是在选择最佳采样时间方面。
